BMRB Entry 4978

Title:
NMR assignments for the Ca2+-bound B0 isoform of the C-terminal globular domain of agrin (agrin-G3)
Deposition date:
2001-03-25
Original release date:
2001-07-30
Authors:
Alexandrescu, Andrei; Maciejewski, Mark; Ruegg, Markus; Engel, Jurgen; Kammerer, Richard
Citation:

Citation: Alexandrescu, Andrei; Maciejewski, Mark; Ruegg, Markus; Engel, Jurgen; Kammerer, Richard. "Letter to the Editor: 1H, 13C and 15N backbone assignments for the C-terminal globular domain of agrin"  J. Biomol. NMR 20, 295-296 (2001).

Assembly members:

Assembly members:
agrin-G3, polymer, 195 residues, 21310 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGSTHis: a derivative of pGEX-1 (Amersham Pharmacia Biotech) that contains a 6xHis tag followed by a thrombin cleavage site between the gluthathione S-transferase (GST) carrier protein and the agrin-G3 domain.

Data sets:
Data typeCount
1H chemical shifts532
13C chemical shifts548
15N chemical shifts193

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1agrin-G31
2Ca 2+2

Entities:

Entity 1, agrin-G3 195 residues - 21310 Da.

1   GLYSERGLULYSVALILEILEGLULYSALA
2   ALAGLYASPALAGLUALAILEALAPHEASP
3   GLYARGTHRTYRMETGLUTYRHISASNALA
4   VALTHRLYSSERGLULYSALALEUGLNSER
5   ASNHISPHEGLULEUSERILELYSTHRGLU
6   ALATHRGLNGLYLEUILELEUTRPSERGLY
7   LYSGLYLEUGLUARGSERASPTYRILEALA
8   LEUALAILEVALASPGLYPHEVALGLNMET
9   METTYRASPLEUGLYSERLYSPROVALVAL
10   LEUARGSERTHRVALPROILEASNTHRASN
11   HISTRPTHRHISILELYSALATYRARGVAL
12   GLNARGGLUGLYSERLEUGLNVALGLYASN
13   GLUALAPROILETHRGLYSERSERPROLEU
14   GLYALATHRGLNLEUASPTHRASPGLYALA
15   LEUTRPLEUGLYGLYMETGLUARGLEUSER
16   VALALAHISLYSLEUPROLYSALATYRSER
17   THRGLYPHEILEGLYCYSILEARGASPVAL
18   ILEVALASPARGGLNGLULEUHISLEUVAL
19   GLUASPALALEUASNASNPROTHRILELEU
20   HISCYSSERALALYS

Entity 2, Ca 2+ - Ca - 40.078 Da.

1   CA

Samples:

sample_1: agrin-G3, [U-13C; U-15N; U-90% 2H], 1.0 mM; CaCl2 4 mM; immidazole 10 mM

sample_2: agrin-G3, [U-15N], 1.0 mM; CaCl2 4 mM; immidazole 10 mM

Ex-Cond_1: pH: 6.5; temperature: 298 K; pressure: 1 atm

Ex-Cond_2: pH: 7.1; temperature: 298 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D HSQC-NOESY-HSQC (200 ms mix time)not availablenot availablenot available
deuterium-decoupled TROSY versions of:not availablenot availablenot available
3D HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
3D 15N TOCSY-HSQC (39 ms mix time)not availablenot availablenot available
3D 15N NOESY-HSQC (100 mix time)not availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks