BMRB Entry 4886

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4886

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H and 15N and 1HB chemical shift assignments for Azotobacter vinelandii C69A apoflavodoxin

Deposition date:

2000-10-26

Original release date:

2000-12-04

Authors:

Steensma, Elles; van Mierlo, Carlo

Citation:

Citation: Steensma, Elles; van Mierlo, Carlo. "Structural characterisation of apoflavodoxin shows that the location of the stable nucleus differs among proteins with a flavodoxin-like topology" J. Mol. Biol. 282, 653-666 (1998).
PubMed: 9737928

Assembly members:

Assembly members:
Azotobacter vinelandii apoflavodoxin II, polymer, 179 residues, 20000 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 354 Superkingdom: Eubacteria Kingdom: not available Genus/species: Azotobacter vinelandii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Azotobacter vinelandii apoflavodoxin II: AKIGLFFGSNTGKTRKVAKS IKKRFDDETMSDALNVNRVS AEDFAQYQFLILGTPTLGEG ELPGLSSDAENESWEEFLPK IEGLDFSGKTVALFGLGDQV GYPENYLDALGELYSFFKDR GAKIVGSWSTDGYEFESSEA VVDGKFVGLALDLDNQSGKT DERVAAWLAQIAPEFGLSL

Data sets:

assigned_chemical_shifts
- shifts_set_1
coupling_constants
- J-values_set_1

Data type	Count
1H chemical shifts	524
15N chemical shifts	146
coupling constants	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C69A apoflavodoxin	1

Entities:

Entity 1, C69A apoflavodoxin 179 residues - 20000 Da.

1

ALA

LYS

ILE

GLY

LEU

PHE

GLY

SER

ASN

2

THR

GLY

LYS

THR

ARG

LYS

VAL

ALA

LYS

SER

3

ILE

LYS

ARG

PHE

ASP

GLU

THR

MET

4

SER

ASP

ALA

LEU

ASN

VAL

ASN

ARG

VAL

SER

5

ALA

GLU

ASP

PHE

ALA

GLN

TYR

GLN

PHE

LEU

6

ILE

LEU

GLY

THR

PRO

THR

LEU

GLY

GLU

GLY

7

GLU

LEU

PRO

GLY

LEU

SER

ASP

ALA

GLU

8

ASN

GLU

SER

TRP

GLU

PHE

LEU

PRO

LYS

9

ILE

GLU

GLY

LEU

ASP

PHE

SER

GLY

LYS

THR

10

VAL

ALA

LEU

PHE

GLY

LEU

GLY

ASP

GLN

VAL

11

GLY

TYR

PRO

GLU

ASN

TYR

LEU

ASP

ALA

LEU

12

GLY

GLU

LEU

TYR

SER

PHE

LYS

ASP

ARG

13

GLY

ALA

LYS

ILE

VAL

GLY

SER

TRP

SER

THR

14

ASP

GLY

TYR

GLU

PHE

GLU

SER

GLU

ALA

15

VAL

ASP

GLY

LYS

PHE

VAL

GLY

LEU

ALA

16

LEU

ASP

LEU

ASP

ASN

GLN

SER

GLY

LYS

THR

17

ASP

GLU

ARG

VAL

ALA

TRP

LEU

ALA

GLN

18

ILE

ALA

PRO

GLU

PHE

GLY

LEU

SER

LEU

Samples:

sample_1: Azotobacter vinelandii apoflavodoxin II, [U-15N], 2 mM; potassium pyrophosphate 150 mM; H2O 90%; D2O 10%

Conditions_sample_1: pH*: 6.0; temperature: 303 K; ionic strength: 0.3 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	not available	Conditions_sample_1
ct-HNHA	sample_1	not available	Conditions_sample_1
ct-HNHB	sample_1	not available	Conditions_sample_1
TOCSY-HMQC	sample_1	not available	Conditions_sample_1
HMQC-NOESY-HSQC	sample_1	not available	Conditions_sample_1
NOESY-HSQC	sample_1	not available	Conditions_sample_1
triple-resonance 5 mm inverse probe with a self-shielded z-gradient	sample_1	not available	Conditions_sample_1

Software:

Felix v2.3 - processing of data

Xeasy - spectral analysis, peak assignment, measurement peak heights

NMR spectrometers:

Bruker AMX 500 MHz

BMRB	15474 17465 4881
PDB	1YOB
GB	AAA22154 AAA64735 ACO76434 AGK13779 AGK18380
PRF	752055A
REF	WP_012698862 YP_002797409 YP_007891221 YP_007896269
SP	P00324
AlphaFold	P00324