BMRB Entry 4784

Title:
1H and 15N chemical shift assignments and interproton 3JHNHA coupling constants of the DNA-binding domain of the tyrosine repressor from Haemophilus influenzae, a transcription factor that belongs to the prokaryotic NtrC superfamily
Deposition date:
2000-07-13
Original release date:
2001-07-17
Authors:
Wang, Yunjun; Zhao, Shimin; Somerville, Ronald; Jardetzky, Oleg
Citation:

Citation: Wang, Yunjun; Zhao, Shimin; Somerville, Ronald; Jardetzky, Oleg. "Solution Structure of the DNA-binding Domain of the TyrR Protein of Haemophilus influenzae"  Protein Sci. 10, 592-598 (2001).

Assembly members:

Assembly members:
repressor, polymer, 61 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Haemophilus influenzae   Taxonomy ID: 727   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Haemophilus influenzae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pZZ257

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts407
15N chemical shifts63
coupling constants34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tyr repressor DNA binding domain1

Entities:

Entity 1, Tyr repressor DNA binding domain 61 residues - Formula weight is not available

1   SERALAVALILESERLEUASPGLUPHEGLU
2   ASNLYSTHRLEUASPGLUILEILEGLYPHE
3   TYRGLUALAGLNVALLEULYSLEUPHETYR
4   ALAGLUTYRPROSERTHRARGLYSLEUALA
5   GLNARGLEUGLYVALSERHISTHRALAILE
6   ALAASNLYSLEULYSGLNTYRGLYILEGLY
7   LYS

Samples:

sample_1: repressor, [U-15N], 2.0 mM

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity plus 800 MHz

Related Database Links:

PDB
EMBL CBW28709 CBY81959 CBY86469
GB AAC22069 ADO80156 ADO95632 AIB45125 EDJ91589
REF NP_438572 WP_005630049 WP_005634169 WP_005652170 WP_005656417
SP P44694
AlphaFold P44694

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks