BMRB Entry 4641

Title:
Human Prion Protein Mutant E200K Fragment 90-231
Deposition date:
2000-08-25
Original release date:
2001-05-17
Authors:
Zhang, Y.; Swietnicki, W.; Zagorski, M.; Surewicz, W.; Soennichsen, F.
Citation:

Citation: Zhang, Y.; Swietnicki, W.; Zagorski, M.; Surewicz, W.; Soennichsen, F.. "Solution structure of Human Prion Protein Mutant E200K Fragment 90-231"  J. Biol. Chem. 275, 33650-33654 (2000).

Assembly members:

Assembly members:
PRION PROTEIN, polymer, 146 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PRSETB

Data sets:
Data typeCount
13C chemical shifts593
1H chemical shifts929
15N chemical shifts162
coupling constants44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PRION PROTEIN1

Entities:

Entity 1, PRION PROTEIN 146 residues - Formula weight is not available

1   GLYSERASPPROGLYGLNGLYGLYGLYTHR
2   HISSERGLNTRPASNLYSPROSERLYSPRO
3   LYSTHRASNMETLYSHISMETALAGLYALA
4   ALAALAALAGLYALAVALVALGLYGLYLEU
5   GLYGLYTYRMETLEUGLYSERALAMETSER
6   ARGPROILEILEHISPHEGLYSERASPTYR
7   GLUASPARGTYRTYRARGGLUASNMETHIS
8   ARGTYRPROASNGLNVALTYRTYRARGPRO
9   METASPGLUTYRSERASNGLNASNASNPHE
10   VALHISASPCYSVALASNILETHRILELYS
11   GLNHISTHRVALTHRTHRTHRTHRLYSGLY
12   GLUASNPHETHRLYSTHRASPVALLYSMET
13   METGLUARGVALVALGLUGLNMETCYSILE
14   THRGLNTYRGLUARGGLUSERGLNALATYR
15   TYRGLNARGGLYSERSER

Samples:

sample_1: PRION PROTEIN, [U-15N; U-13C], 0.8 mM; acetate buffer 10 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 4.6; temperature: 299 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablesample_cond_1
3D 15N-separated NOESYsample_1not availablesample_cond_1
2D NOESYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1

Software:

NMRPipe vsgi6x.m4 - processing

FELIX v98 - processing

PIPP v4.24 - data analysis

ARIA v0.9 - structure solution

CNS v0.9 - structure solution, refinement

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15676 16743 16757 17714 17756 17757 17780 18426 18550 19268 4379 4402 4434 4620 4736
PDB
DBJ BAF62360 BAG32276 BAG32278 BAG52189 BAG56832
EMBL CAA58442 CAG46836
GB AAA60182 AAA68632 AAB59442 AAB59443 AAC05365
REF NP_000302 NP_001009093 NP_001073590 NP_001073591 NP_001073592
SP P04156 P61766 P61767 P61768
AlphaFold P61768 P61767 P61766 P04156

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks