BMRB Entry 4436

Title:
Proton Assignment of the Ferrocytochrome C3 from Desulfovibrio gigas.
Deposition date:
1999-10-12
Original release date:
2000-12-07
Authors:
Brennan, L.; Turner, D.; Messias, A.; Teodoro, M.; Legall, J.; Santos, H.; Xavier, A.
Citation:

Citation: Brennan, L.; Turner, D.; Messias, A.; Teodoro, M.; Legall, J.; Santos, H.; Xavier, A.. "Structural Basis for the Network of Functional Cooperativities in Cytochrome c(3) from Desulfovibrio gigas: Solution Structures of the Oxidised and Reduced states"  J. Mol. Biol. 298, 61-82 (2000).

Assembly members:

Assembly members:
CYTOCHROME C3, polymer, 112 residues, 14000 Da.
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 879   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Desulfovibrio gigas

Experimental source:

Experimental source:   Production method: purified from the natural source

Data typeCount
1H chemical shifts808
coupling constants67

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cyt c3 peptide1
2heme I2
3heme II2
4heme III2
5heme IV2

Entities:

Entity 1, cyt c3 peptide 112 residues - 14000 Da.

1   VALASPVALPROALAASPGLYALALYSILE
2   ASPPHEILEALAGLYGLYGLULYSASNLEU
3   THRVALVALPHEASNHISSERTHRHISLYS
4   ASPVALLYSCYSASPASPCYSHISHISGLN
5   PROGLYASPLYSGLNTYRALAGLYCYSTHR
6   THRASPGLYCYSHISASNILELEUASPLYS
7   ALAASPLYSSERVALASNSERTRPTYRLYS
8   VALVALHISASPALALYSGLYGLYALALYS
9   PROTHRCYSILESERCYSHISLYSASPLYS
10   ALAGLYASPASPLYSGLULEULYSLYSLYS
11   LEUTHRGLYCYSLYSGLYSERALACYSHIS
12   PROSER

Entity 2, heme I - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_one: CYTOCHROME C3 3.2 mM; ampicillin 70 uM; kanamicine 50 uM; chloramphenicol 50 uM

sample_conditions: pH: 7.3 na; temperature: 303.0 K

Experiments:

NameSampleSample stateSample conditions
2D-1H-NOESYsample_onenot availablesample_conditions
2D-1H-TOCSYsample_onenot availablesample_conditions
2D-1H-COSYsample_onenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

BMRB 4435
PDB
EMBL CAB59196
GB AGW12081
REF WP_021758659
SP P00133
AlphaFold P00133