BMRB Entry 4168

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the Receptor Binding Domain of Human Alpha-2-macroglobulin
Deposition date:
1998-07-24
Original release date:
2000-03-27
Authors:
Huang, Wen; Dolmer, Klavs; Liao, Xiubei; Gettins, Peter
Citation:

Citation: Huang, Wen; Dolmer, Klavs; Liao, Xiubei; Gettins, Peter. "Localization of Basic Residues Required for Receptor Binding to the Single Alpha-helix of the Receptor Binding Domain of Human Alpha-2-macroglobulin"  Protein Sci. 7, 2602-2612 (1998).

Assembly members:

Assembly members:
Alpha-2-macroglobulin receptor binding domain, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts138
15N chemical shifts130
1H chemical shifts271

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RBD1

Entities:

Entity 1, RBD 138 residues - Formula weight is not available

1   GLUGLUPHEPROPHEALALEUGLYVALGLN
2   THRLEUPROGLNTHRCYSASPGLUPROLYS
3   ALAHISTHRSERPHEGLNILESERLEUSER
4   VALSERTYRTHRGLYSERARGSERALASER
5   ASNMETALAILEVALASPVALLYSMETVAL
6   SERGLYPHEILEPROLEULYSPROTHRVAL
7   LYSMETLEUGLUARGSERASNHISVALSER
8   ARGTHRGLUVALSERSERASNHISVALLEU
9   ILETYRLEUASPLYSVALSERASNGLNTHR
10   LEUSERLEUPHEPHETHRVALLEUGLNASP
11   VALPROVALARGASPLEULYSPROALAILE
12   VALLYSVALTYRASPTYRTYRGLUTHRASP
13   GLUPHEALAILEALAGLUTYRASNALAPRO
14   CYSSERLYSASPLEUGLYASNALA

Samples:

sample_one: Alpha-2-macroglobulin receptor binding domain, [U-95% 13C; U-90%15N], 0.9 – 1.2 mM

sample_conditions_one: ionic strength: 0.4 M; pH: 5.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_onenot availablenot available
1H-15N TOCSYsample_onenot availablenot available
NOESY-HSQCsample_onenot availablenot available
HNCAsample_onenot availablenot available
HNCACBsample_onenot availablenot available
CBCA(CO)NHsample_onenot availablenot available
HBHA(CO)NHsample_onenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAD92851 BAG10847
EMBL CAH18188 CAH93278
GB AAA51551 AAA51552 AAH40071 AAQ13498 AAT02228
PRF 1009174A
REF NP_000005 NP_001126929 XP_001139819 XP_003265499 XP_003804581
SP P01023 Q5R4N8
AlphaFold P01023 Q5R4N8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks