BMRB Entry 4081

Title:
Sequence-specific 1H, 13C and 15N NMR Assignments of Recombinant Human Interferon Alpha-2a
Deposition date:
1997-12-15
Original release date:
1998-03-20
Authors:
Werner, Klaus; Grzesiek, Stephan; Labhardt, Alexander; Senn, Hans
Citation:

Citation: Werner, Klaus; Gsell, Bernard; Labhardt, Alexander; Wipf, Beat; Senn, Hans. "The Three-dimensional High Resolution Structure of Human Interferon alpha-2a Determined by Hetero-nuclear NMR Spectroscopy in Solution"  J. Mol. Biol. 274, 661-675 (1997).

Assembly members:

Assembly members:
Interferon_alpha-2a, polymer, 165 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts724
15N chemical shifts172
1H chemical shifts1193

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1INF-alpha-2a1

Entities:

Entity 1, INF-alpha-2a 165 residues - Formula weight is not available

1   CYSASPLEUPROGLNTHRHISSERLEUGLY
2   SERARGARGTHRLEUMETLEULEUALAGLN
3   METARGLYSILESERLEUPHESERCYSLEU
4   LYSASPARGHISASPPHEGLYPHEPROGLN
5   GLUGLUPHEGLYASNGLNPHEGLNLYSALA
6   GLUTHRILEPROVALLEUHISGLUMETILE
7   GLNGLNILEPHEASNLEUPHESERTHRLYS
8   ASPSERSERALAALATRPASPGLUTHRLEU
9   LEUASPLYSPHETYRTHRGLULEUTYRGLN
10   GLNLEUASNASPLEUGLUALACYSVALILE
11   GLNGLYVALGLYVALTHRGLUTHRPROLEU
12   METLYSGLUASPSERILELEUALAVALARG
13   LYSTYRPHEGLNARGILETHRLEUTYRLEU
14   LYSGLULYSLYSTYRSERPROCYSALATRP
15   GLUVALVALARGALAGLUILEMETARGSER
16   PHESERLEUSERTHRASNLEUGLNGLUSER
17   LEUARGSERLYSGLU

Samples:

sample_one: Interferon_alpha-2a 2.0 mM

sample_conditions: pH: 3.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D_1H-1H_HOHAHAsample_onenot availablesample_conditions
2D_1H-1H_NOESYsample_onenot availablesample_conditions
2D_1H-1H_CLEAN-TOCSYsample_onenot availablesample_conditions
2D_1H-13C_HSQCsample_onenot availablesample_conditions
2D_1H-13C_CT-HSQCsample_onenot availablesample_conditions
2D_1H-15N_HSQCsample_onenot availablesample_conditions
Three-dimensional experimentssample_onenot availablesample_conditions

Software:

NMRPipe -

NMR spectrometers:

  • Bruker AMX500 500 MHz
  • Bruker AMX2-500 500 MHz
  • Bruker DMX600 600 MHz

Related Database Links:

BMRB 16677 18135 18344
PDB
DBJ BAJ20902
EMBL CAA23805 CAA23809 CAA23810 CAA25770 CAA46954
GB AAA52715 AAA59181 AAB59402 AAC64915 AAH74936
PRF 0611561A 0906342A
REF NP_000596 XP_003830724 XP_004047919 XP_528568
SP P01563
AlphaFold P01563

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks