BMRB Entry 27845

Title:
Solid-state NMR backbone assignment of HBV core protein at 100 kHz
Deposition date:
2019-03-20
Original release date:
2019-08-14
Authors:
Lecoq, Lauriane; Schledorn, Maarten; Wang, Shishan; Smith-Penzel, Susanne; Malar, Alexander; Callon, Morgane; Nassal, Michael; Meier, Beat; Bockmann, Anja
Citation:

Citation: Lecoq, Lauriane; Schledorn, Maarten; Wang, Shishan; Smith-Penzel, Susanne; Malar, Alexander; Callon, Morgane; Nassal, Michael; Meier, Beat; Bockmann, Anja. "100 kHz MAS Proton-Detected NMR Spectroscopy of Hepatitis B Virus Capsids"  Front. Mol. Biosci. 6, .-. (2019).
PubMed: 31396521

Assembly members:

Assembly members:
Cp149, polymer, 149 residues, 17777 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a2

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts131
1H chemical shifts134

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cp1491

Entities:

Entity 1, Cp149 149 residues - 17777 Da.

1   METASPILEASPPROTYRLYSGLUPHEGLY
2   ALATHRVALGLULEULEUSERPHELEUPRO
3   SERASPPHEPHEPROSERVALARGASPLEU
4   LEUASPTHRALASERALALEUTYRARGGLU
5   ALALEUGLUSERPROGLUHISCYSSERPRO
6   HISHISTHRALALEUARGGLNALAILELEU
7   CYSTRPGLYGLULEUMETTHRLEUALATHR
8   TRPVALGLYVALASNLEUGLUASPPROALA
9   SERARGASPLEUVALVALSERTYRVALASN
10   THRASNMETGLYLEULYSPHEARGGLNLEU
11   LEUTRPPHEHISILESERCYSLEUTHRPHE
12   GLYARGGLUTHRVALILEGLUTYRLEUVAL
13   SERPHEGLYVALTRPILEARGTHRPROPRO
14   ALATYRARGPROPROASNALAPROILELEU
15   SERTHRLEUPROGLUTHRTHRVALVAL

Samples:

protonated_Cp149: Cp149, [U-100% 13C; U-100% 15N], 30 mg/mL; TRIS 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D hNHprotonated_Cp149isotropicsample_conditions_1
3D hCANHprotonated_Cp149isotropicsample_conditions_1
3D hCONHprotonated_Cp149isotropicsample_conditions_1
3D HNcoCAprotonated_Cp149isotropicsample_conditions_1
3D hncaCBCANHprotonated_Cp149isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CcpNmr v2.4.2, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 850 MHz

Related Database Links:

UNP P03146
BMRB 27317
AlphaFold P03146

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks