Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27798
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Citation: Liess, Anna; Kucerova, Alena; Schweimer, Kristian; Yu, Lu; Roumeliotis, Theodoros; Diebold, Mathias; Dybkov, Olexandr; Sotriffer, Christoph; Urlaub, Henning; Choudhary, Jyoti; Mansfeld, Jorg; Lorenz, Sonja. "Autoinhibition Mechanism of the Ubiquitin-Conjugating Enzyme UBE2S by Autoubiquitination" Structure 27, 1195-1210 (2019).
PubMed: 31230944
Assembly members:
UBE2S, polymer, 156 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: modified pSKB2 vector
Data type | Count |
13C chemical shifts | 423 |
15N chemical shifts | 137 |
1H chemical shifts | 137 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | UBE2S UBC | 1 |
Entity 1, UBE2S UBC 156 residues - Formula weight is not available
1 | MET | ASN | SER | ASN | VAL | GLU | ASN | LEU | PRO | PRO | ||||
2 | HIS | ILE | ILE | ARG | LEU | VAL | TYR | LYS | GLU | VAL | ||||
3 | THR | THR | LEU | THR | ALA | ASP | PRO | PRO | ASP | GLY | ||||
4 | ILE | LYS | VAL | PHE | PRO | ASN | GLU | GLU | ASP | LEU | ||||
5 | THR | ASP | LEU | GLN | VAL | THR | ILE | GLU | GLY | PRO | ||||
6 | GLU | GLY | THR | PRO | TYR | ALA | GLY | GLY | LEU | PHE | ||||
7 | ARG | MET | LYS | LEU | LEU | LEU | GLY | LYS | ASP | PHE | ||||
8 | PRO | ALA | SER | PRO | PRO | LYS | GLY | TYR | PHE | LEU | ||||
9 | THR | LYS | ILE | PHE | HIS | PRO | ASN | VAL | GLY | ALA | ||||
10 | ASN | GLY | GLU | ILE | SER | VAL | ASN | VAL | LEU | CYS | ||||
11 | ARG | ASP | TRP | THR | ALA | GLU | LEU | GLY | ILE | ARG | ||||
12 | HIS | VAL | LEU | LEU | THR | ILE | LYS | MET | LEU | LEU | ||||
13 | ILE | HIS | PRO | ASN | PRO | GLU | SER | ALA | LEU | ASN | ||||
14 | GLU | GLU | ALA | GLY | ARG | LEU | LEU | LEU | GLU | ASN | ||||
15 | TYR | GLU | GLU | TYR | ALA | ALA | ARG | ALA | ARG | LEU | ||||
16 | LEU | THR | GLU | ILE | HIS | GLY |
sample_1: UBE2S, [U-100% 13C; U-100% 15N], 0.6 mM; D2O 5%; sodium phosphate 75 mM; DTT 5 mM; EDTA 1 mM; TCEP 2 mM
sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
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