BMRB Entry 27726
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27726
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Li, Yifei; Soubias, Olivier; Li, Jess; Sun, Shangjin; Randazzo, Paul; Byrd, R Andrew. "Functional Expression and Characterization of Human Myristoylated-Arf1 in Nanodisc Membrane Mimetics." Biochemistry 58, 1423-1431 (2019).
PubMed: 30735034
Assembly members:
Arf1_L8K, polymer, 181 residues, 20711.78 Da.
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE, non-polymer, 539.246 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET19b
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 308 |
| 15N chemical shifts | 130 |
| 1H chemical shifts | 355 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Arf1_L8K | 1 |
| 2 | GTPgS | 2 |
Entities:
Entity 1, Arf1_L8K 181 residues - 20711.78 Da.
| 1 | MET | GLY | ASN | ILE | PHE | ALA | ASN | LYS | PHE | LYS | ||||
| 2 | GLY | LEU | PHE | GLY | LYS | LYS | GLU | MET | ARG | ILE | ||||
| 3 | LEU | MET | VAL | GLY | LEU | ASP | ALA | ALA | GLY | LYS | ||||
| 4 | THR | THR | ILE | LEU | TYR | LYS | LEU | LYS | LEU | GLY | ||||
| 5 | GLU | ILE | VAL | THR | THR | ILE | PRO | THR | ILE | GLY | ||||
| 6 | PHE | ASN | VAL | GLU | THR | VAL | GLU | TYR | LYS | ASN | ||||
| 7 | ILE | SER | PHE | THR | VAL | TRP | ASP | VAL | GLY | GLY | ||||
| 8 | GLN | ASP | LYS | ILE | ARG | PRO | LEU | TRP | ARG | HIS | ||||
| 9 | TYR | PHE | GLN | ASN | THR | GLN | GLY | LEU | ILE | PHE | ||||
| 10 | VAL | VAL | ASP | SER | ASN | ASP | ARG | GLU | ARG | VAL | ||||
| 11 | ASN | GLU | ALA | ARG | GLU | GLU | LEU | MET | ARG | MET | ||||
| 12 | LEU | ALA | GLU | ASP | GLU | LEU | ARG | ASP | ALA | VAL | ||||
| 13 | LEU | LEU | VAL | PHE | ALA | ASN | LYS | GLN | ASP | LEU | ||||
| 14 | PRO | ASN | ALA | MET | ASN | ALA | ALA | GLU | ILE | THR | ||||
| 15 | ASP | LYS | LEU | GLY | LEU | HIS | SER | LEU | ARG | HIS | ||||
| 16 | ARG | ASN | TRP | TYR | ILE | GLN | ALA | THR | CYS | ALA | ||||
| 17 | THR | SER | GLY | ASP | GLY | LEU | TYR | GLU | GLY | LEU | ||||
| 18 | ASP | TRP | LEU | SER | ASN | GLN | LEU | ARG | ASN | GLN | ||||
| 19 | LYS |
Entity 2, GTPgS - C10 H16 N5 O13 P3 S - 539.246 Da.
| 1 | GSP |
Samples:
sample_1: Arf1_L8K_GTPgS, [U-100% 13C; U-100% 15N; U-95% 2H] ILV methyl protonated, 500 uM
sample_2: Arf1_L8K_GTPgS, [U-100% 15N; U-95% 2H] ILV methyl 13C labeled and protonated, 500 uM
sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_2 | isotropic | sample_conditions_1 |
| 3D (H)CC(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 4D methyl-methyl NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, collection, data analysis, peak picking, processing
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks