BMRB Entry 27613

Title:
1H, 15N and 13C backbone assignment of apoTDP-43 RNA Recognition Motifs
Deposition date:
2018-09-14
Original release date:
2018-09-25
Authors:
Scott, David; Kumirov, Vlad; Francois-Moutal, Liberty; Khanna, May
Citation:

Citation: Scott, David; Francois-Moutal, Liberty; Kumirov, Vlad; Khanna, May. "1H, 15N and 13C backbone assignment of apo TDP-43 RNA recognition motifs"  Biomol. NMR Assignments 13, 163-167 (2019).
PubMed: 30694439

Assembly members:

Assembly members:
TDP43, polymer, 202 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Data sets:
Data typeCount
13C chemical shifts435
15N chemical shifts142
1H chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM, chain 11
2RRM, chain 21

Entities:

Entity 1, RRM, chain 1 202 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALASERMETTHRGLYGLYGLNGLNMET
4   GLYARGGLYSERLYSTHRSERASPLEUILE
5   VALLEUGLYLEUPROTRPLYSTHRTHRGLU
6   GLNASPLEULYSGLUTYRPHESERTHRPHE
7   GLYGLUVALLEUMETVALGLNVALLYSLYS
8   ASPLEULYSTHRGLYHISSERLYSGLYPHE
9   GLYPHEVALARGPHETHRGLUTYRGLUTHR
10   GLNVALLYSVALMETSERGLNARGHISMET
11   ILEASPGLYARGTRPCYSASPCYSLYSLEU
12   PROASNSERLYSGLNSERGLNASPGLUPRO
13   LEUARGSERARGLYSVALPHEVALGLYARG
14   CYSTHRGLUASPMETTHRGLUASPGLULEU
15   ARGGLUPHEPHESERGLNTYRGLYASPVAL
16   METASPVALPHEILEPROLYSPROPHEARG
17   ALAPHEALAPHEVALTHRPHEALAASPASP
18   GLNILEALAGLNSERLEUCYSGLYGLUASP
19   LEUILEILELYSGLYILESERVALHISILE
20   SERASNALAGLUPROLYSHISASNSERASN
21   ARGGLN

Samples:

sample_1: TDP43, [U-100% 13C; U-100% 15N], 450 uM; DTT 4 mM; sodium azide 0.5%; HEPES 40 mM; sodium chloride 500 mM; D2O 10%

sample_conditions_1: ionic strength: 0.5 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY v3.134, Goddard - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks