BMRB Entry 27361

Title:
Stromal Interaction Molecule 1 CC1 R304W mutant
Deposition date:
2018-01-15
Original release date:
2018-01-22
Authors:
Fahrner, Marc; Stadlbauer, Michael; Muik, Martin; Rathner, Petr; Stathopulos, Peter; Ikura, Mitsuhiko; Mueller, Norbert; Romanin, Christoph
Citation:

Citation: Fahrner, Marc; Stadlbauer, Michael; Muik, Martin; Rathner, Petr; Stathopulos, Peter; Ikura, Mitsuhiko; Mueller, Norbert; Romanin, Christoph. "A novel mechanism promotes switching of the Stormorken STIM1 R304W mutant into the activated state"  Nat. Comm. ., .-..

Assembly members:

Assembly members:
STIM1_CC1_R304W, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 4T-1

Data sets:
Data typeCount
15N chemical shifts10
1H chemical shifts16
T1 relaxation values10

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CC11

Entities:

Entity 1, CC1 115 residues - Formula weight is not available

1   GLYSERPROGLUPHEASNARGTYRSERLYS
2   GLUHISMETLYSLYSMETMETLYSASPLEU
3   GLUGLYLEUHISARGALAGLUGLNSERLEU
4   HISASPLEUGLNGLUARGLEUHISLYSALA
5   GLNGLUGLUHISARGTHRVALGLUVALGLU
6   LYSVALHISLEUGLULYSLYSLEUARGASP
7   GLUILEASNLEUALALYSGLNGLUALAGLN
8   ARGLEULYSGLULEUTRPGLUGLYTHRGLU
9   ASNGLUARGSERARGGLNLYSTYRALAGLU
10   GLUGLULEUGLUGLNVALARGGLUALALEU
11   ARGLYSALAGLULYSGLULEUGLUSERHIS
12   SERSERTRPTYRALA

Samples:

sample_1: STIM1_CC1_R304W, [U-98% 13C; U-98% 15N], 3 mM; H2O 90%; sodium phosphate 20 mM; SDS, [U-99% 2H], 7 mM; L-Arginine 50 mM; L-Glutamic acid 50 mM; sodium chloride 300 mM; D2O, [U-99% 2H], 10%

sample_conditions_1: ionic strength: 0.685 M; pH: 8.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D (H)C(CCO)NHsample_1isotropicsample_conditions_1
3D TOCSY-HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.2, 3.5, Bruker Biospin, Keller and Wuthrich - chemical shift assignment, collection

NMR spectrometers:

  • Bruker Avance III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks