BMRB Entry 27348

Title:
1H, 13C and 15N assignment of C', CA, N and HN of intrinsically disordered alpha-synuclein near-phsysiological conditions
Deposition date:
2017-12-22
Original release date:
2018-03-08
Authors:
Schiavina, Marco; Murrali, Maria Grazia; Sainati, Valerio; Bermel, Wolfgang; Pierattelli, Roberta; Felli, Isabella
Citation:

Citation: Murrali, Maria Grazia; Schiavina, Marco; Sainati, Valerio; Bermel, Wolfgang; Pierattelli, Roberta; Felli, Isabella. "13C APSY-NMR for sequential assignment of intrinsically disordered proteins"  J. Biomol. NMR 70, 167-175 (2018).
PubMed: 29492731

Assembly members:

Assembly members:
intrinsically_disordered_alpha-synuclein, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pT7-7

Data sets:
Data typeCount
13C chemical shifts273
15N chemical shifts139
1H chemical shifts93

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1intrinsically disordered alpha-synuclein1

Entities:

Entity 1, intrinsically disordered alpha-synuclein 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAALA
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: intrinsically disordered alpha-synuclein, [U-13C; U-15N], 1.0 mM; KPI 20 mM; EDTA 0.5 mM; NaCl 200 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 6.5; temperature: 315 K

Experiments:

NameSampleSample stateSample conditions
5D APSY-(HCA)CONCACONsample_1isotropicsample_conditions_1
3D APSY-HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D CONsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.0.1, Bruker Biospin - chemical shift assignment

NMR spectrometers:

  • Bruker Avance NEO 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks