BMRB Entry 27344

Title:
Backbone 1H, 13C, 15N Chemical Shift Assignments of GA Seq5
Deposition date:
2017-12-19
Original release date:
2018-03-08
Authors:
Tian, Pengfei; Louis, John; Baber, James; Best, Robert
Citation:

Citation: Tian, Pengfei; Louis, John; Baber, James; Aniana, A.; Best, Robert. "Co-evolutionary fitness landscapes for sequence design"  Angew. Chem. Int. Ed. Engl. 57, 5674-5678 (2018).
PubMed: 29512300

Assembly members:

Assembly members:
GA_Seq5, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: na

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts115
15N chemical shifts57
1H chemical shifts199
residual dipolar couplings33

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GA Seq5 monomer1

Entities:

Entity 1, GA Seq5 monomer 68 residues - Formula weight is not available

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYMETGLUSERALALYSALAALAGLU
3   LEUALAGLUALALYSLYSALAALALEUASP
4   GLUPHEGLULYSTYRGLYVALSERASPTYR
5   PHELYSLYSLEUVALGLUASNALALYSTHR
6   VALASPGLYVALTRPALALEUGLNALAGLN
7   VALVALGLUSERLEUPROTHRGLU

Samples:

sample_1: GA Seq5, [U-99% 13C; U-99% 15N], 0.67 mM; sodium phosphate 50 mM

sample_2: GA Seq5, [U-99% 13C; U-99% 15N], 0.67 mM; sodium phosphate 50 mM; pf1 phage 13 mg/mL

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 2 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQC IPAPsample_2anisotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

ccpnmr, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks