BMRB Entry 27124

Title:
hnRNPA2 D290V low complexity domain 190-341 backbone and Cbeta chemical shifts
Deposition date:
2017-06-05
Original release date:
2018-01-11
Authors:
Ryan, Veronica; Fawzi, Nicolas; Silva, Rute; Burke, Kathleen
Citation:

Citation: Ryan, Veronica; Dignon, Gregory; Zerze, Gul; Chabata, Charlene; Silva, Rute; Conicella, Alexander; Amaya, Joshua; Burke, Kathleen; Mittal, Jeetain; Fawzi, Nicolas. "Disease mutation, protein interactions, and posttranslational modifications modulate hnRNPA2 liquid-liquid phase separation"  Mol. Cell 69, 465-479 (2018).
PubMed: 29358076

Assembly members:

Assembly members:
hnRNPA2_LC_D290V, polymer, 155 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: hnRNPA2_LC_D290V_pJ411

Data sets:
Data typeCount
13C chemical shifts359
15N chemical shifts144
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hnRNPA2 LC D290V1

Entities:

Entity 1, hnRNPA2 LC D290V 155 residues - Formula weight is not available

Resides 1-3 are cloning and cleavage remnants, residue 4 is residue 190 in human hnRNPA2 LC

1   GLYHISMETGLYARGGLYGLYASNPHEGLY
2   PHEGLYASPSERARGGLYGLYGLYGLYASN
3   PHEGLYPROGLYPROGLYSERASNPHEARG
4   GLYGLYSERASPGLYTYRGLYSERGLYARG
5   GLYPHEGLYASPGLYTYRASNGLYTYRGLY
6   GLYGLYPROGLYGLYGLYASNPHEGLYGLY
7   SERPROGLYTYRGLYGLYGLYARGGLYGLY
8   TYRGLYGLYGLYGLYPROGLYTYRGLYASN
9   GLNGLYGLYGLYTYRGLYGLYGLYTYRASP
10   ASNTYRGLYGLYGLYASNTYRGLYSERGLY
11   ASNTYRASNVALPHEGLYASNTYRASNGLN
12   GLNPROSERASNTYRGLYPROMETLYSSER
13   GLYASNPHEGLYGLYSERARGASNMETGLY
14   GLYPROTYRGLYGLYGLYASNTYRGLYPRO
15   GLYGLYSERGLYGLYSERGLYGLYTYRGLY
16   GLYARGSERARGTYR

Samples:

sample_1: hnRNPA2 LC D290V, [U-99% 13C; U-99% 15N], 100 uM; D2O, [U-2H], 10%; MES 20 mM; Bis Tris 1 ± 1 mM; H2O 90%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HN(CAN)NHsample_1isotropicsample_conditions_1

Software:

CARA, Wuthrich - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks