BMRB Entry 26852

Title:
Partial Backbone 1H, 13C, and 15N Chemical Shift Assignments for VirB8 from Brucella suis
Deposition date:
2016-07-13
Original release date:
2017-02-22
Authors:
Sharifahmadian, Mahzad; Lecoq, Lauriane; Omichinski, James; Baron, Christian
Citation:

Citation: Sharifahmadian, Mahzad; Arya, Tarun; Bessette, Benoit; Lecoq, Lauriane; Ruediger, Edward; Omichinski, James; Baron, Christian. "Monomer-to-dimer transition of Brucella suis type IV secretion system component VirB8 induces conformational changes"  FEBS J. 284, 1218-1232 (2017).
PubMed: 28236662

Assembly members:

Assembly members:
VirB8p, polymer, 164 residues, 18202.1504 Da.

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHTVirB8sp

Data sets:
Data typeCount
13C chemical shifts416
15N chemical shifts146
1H chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VirB8p subunit, 11
2VirB8p subunit, 21

Entities:

Entity 1, VirB8p subunit, 1 164 residues - 18202.1504 Da.

1   ALAARGVALASNALAGLNTHRGLYALAPRO
2   ASPILELEUTHRSERLEUASPGLULYSSER
3   VALSERTYRASPTHRVALMETASPLYSTYR
4   TRPLEUSERGLNTYRVALILEALAARGGLU
5   THRTYRASPTRPTYRTHRLEUGLNLYSASP
6   TYRGLUTHRVALGLYMETLEUSERSERPRO
7   SERGLUGLYGLNSERTYRALASERGLNPHE
8   GLNGLYASPLYSALALEUASPLYSGLNTYR
9   GLYSERASNVALARGTHRSERVALTHRILE
10   VALSERILEVALPROASNGLYLYSGLYILE
11   GLYTHRVALARGPHEALALYSTHRTHRLYS
12   ARGTHRASNGLUTHRGLYASPGLYGLUTHR
13   THRHISTRPILEALATHRILEGLYTYRGLN
14   TYRVALASNPROSERLEUMETSERGLUSER
15   ALAARGLEUTHRASNPROLEUGLYPHEASN
16   VALTHRSERTYRARGVALASPPROGLUMET
17   GLYVALVALGLN

Samples:

sample_1: Molecule_VirB8p, [U-13C; U-15N], 0.35 mM; sodium phosphate 20 mM; sodium chloride 100 mM; D2O, [U-99% 2H], 10%; H2O 90%; CHAPS 30 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks