BMRB Entry 26846

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26846

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Title:
Backbone assignments of the full length Dengue Virus NS4B protein in micelles
Deposition date:
2016-07-07
Original release date:
2018-06-26
Authors:
Li, Yan; Wong, Ying Lei; Lee, Michelle Yueqi; Kang, CongBao
Citation:

Citation: Li, Yan; Wong, Ying Lei; Lee, Michelle Yueqi; Li, Qingxin; Wang, Qing-Yin; Lescar, Julien; Shi, Pei-Yong; Kang, CongBao. "Secondary Structure and Membrane Topology of the Full-Length Dengue Virus NS4B in Micelles."  Angew. Chem. Int. Ed. Engl. 55, 12068-12072 (2016).
PubMed: 27554985

Assembly members:

Assembly members:
full_length_Dengue_NS4B, polymer, 257 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: viruses   Taxonomy ID: 12637   Superkingdom: Viruses   Kingdom: Flavivirus   Genus/species: Dengue Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
full_length_Dengue_NS4B: MNEMGLLETTKRDLGMSKEP GVASPTSYLDVDLHPASAWT LYAVATTVITPMLRHTIENS TANVSLAAIANQAVVLMGLD KGWPISKMDLGVPLLALGCY SQVNPLTLTAAVLLLVTHYA IIGPGLQAKATREAQKRTAA GIMKNPTVDGIMTIDLDPVI YDSKFEKQLGQVMLLVLCAV QLLLMRTSWAFCEALTLATG PITTLWEGSPGKFWNTTIAV SMANIFRGSYLAGAGLAFSI MKSVGTGKRLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts699
15N chemical shifts230
1H chemical shifts229

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1full length Dengue NS4B1

Entities:

Entity 1, full length Dengue NS4B 257 residues - Formula weight is not available

1   METASNGLUMETGLYLEULEUGLUTHRTHR
2   LYSARGASPLEUGLYMETSERLYSGLUPRO
3   GLYVALALASERPROTHRSERTYRLEUASP
4   VALASPLEUHISPROALASERALATRPTHR
5   LEUTYRALAVALALATHRTHRVALILETHR
6   PROMETLEUARGHISTHRILEGLUASNSER
7   THRALAASNVALSERLEUALAALAILEALA
8   ASNGLNALAVALVALLEUMETGLYLEUASP
9   LYSGLYTRPPROILESERLYSMETASPLEU
10   GLYVALPROLEULEUALALEUGLYCYSTYR
11   SERGLNVALASNPROLEUTHRLEUTHRALA
12   ALAVALLEULEULEUVALTHRHISTYRALA
13   ILEILEGLYPROGLYLEUGLNALALYSALA
14   THRARGGLUALAGLNLYSARGTHRALAALA
15   GLYILEMETLYSASNPROTHRVALASPGLY
16   ILEMETTHRILEASPLEUASPPROVALILE
17   TYRASPSERLYSPHEGLULYSGLNLEUGLY
18   GLNVALMETLEULEUVALLEUCYSALAVAL
19   GLNLEULEULEUMETARGTHRSERTRPALA
20   PHECYSGLUALALEUTHRLEUALATHRGLY
21   PROILETHRTHRLEUTRPGLUGLYSERPRO
22   GLYLYSPHETRPASNTHRTHRILEALAVAL
23   SERMETALAASNILEPHEARGGLYSERTYR
24   LEUALAGLYALAGLYLEUALAPHESERILE
25   METLYSSERVALGLYTHRGLYLYSARGLEU
26   GLUHISHISHISHISHISHIS

Samples:

sample_1: full length Dengue NS4B, [U-13C; U-15N; U-2H], 0.8 mM; sodium phosphate 20 mM; LMPG 1%; DTT 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin, Goddard, Johnson, One Moon Scientific, Keller and Wuthrich - chemical shift assignment, collection

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks