BMRB Entry 26644

Title:
Backbone assignment of the N-terminal 24-kDa fragment of Escherichia coli topoisomerase IV ParE subunit
Deposition date:
2015-08-31
Original release date:
2015-12-17
Authors:
Li, Yan; Wong, Ying Lei; Lee, Michelle Yueqi; Ng, Hui Qi; Kang, CongBao
Citation:

Citation: Li, Yan; Wong, Ying Lei; Lee, Michelle Yueqi; Ng, Hui Qi; Kang, CongBao. "Backbone assignment of the N-terminal 24-kDa fragment of Escherichia coli topoisomerase IV ParE subunit"  Biomol. NMR Assignments 10, 135-138 (2016).
PubMed: 26482923

Assembly members:

Assembly members:
N-terminal_24-kDa_fragment_of_Escherichia_coli_topoisomerase_IV_ParE_subunit, polymer, 226 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET29b

Data sets:
Data typeCount
13C chemical shifts610
15N chemical shifts203
1H chemical shifts203

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal 24-kDa fragment of Escherichia coli topoisomerase IV ParE subunit1

Entities:

Entity 1, N-terminal 24-kDa fragment of Escherichia coli topoisomerase IV ParE subunit 226 residues - Formula weight is not available

1   METTHRGLNTHRTYRASNALAASPALAILE
2   GLUVALLEUTHRGLYLEUGLUPROVALARG
3   ARGARGPROGLYMETTYRTHRASPTHRTHR
4   ARGPROASNHISLEUGLYGLNGLUVALILE
5   ASPASNSERVALASPGLUALALEUALAGLY
6   HISALALYSARGVALASPVALILELEUHIS
7   ALAASPGLNSERLEUGLUVALILEASPASP
8   GLYARGGLYMETPROVALASPILEHISPRO
9   GLUGLUGLYVALPROALAVALGLULEUILE
10   LEUCYSARGLEUHISALAGLYGLYLYSPHE
11   SERASNLYSASNTYRGLNPHESERGLYGLY
12   LEUHISGLYVALGLYILESERVALVALASN
13   ALALEUSERLYSARGVALGLUVALASNVAL
14   ARGARGASPGLYGLNVALTYRASNILEALA
15   PHEGLUASNGLYGLULYSVALGLNASPLEU
16   GLNVALVALGLYTHRCYSGLYLYSARGASN
17   THRGLYTHRSERVALHISPHETRPPROASP
18   GLUTHRPHEPHEASPSERPROARGPHESER
19   VALSERARGLEUTHRHISVALLEULYSALA
20   LYSALAVALLEUCYSPROGLYVALGLUILE
21   THRPHELYSASPGLUILEASNASNTHRGLU
22   GLNARGTRPCYSTYRGLNASPGLYLEUGLU
23   HISHISHISHISHISHIS

Samples:

sample_1: Escherichia coli active domains of the type II topoisomerases, [U-100% 13C; U-100% 15N; U-70% 2H], 0.6 mM; sodium phosphate 20 mM; potassium chloride 80 mM; DTT 2 mM; EDTA 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific, Keller and Wuthrich - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks