BMRB Entry 26607

Title:
Neisseria meningititis Fic
Deposition date:
2015-07-13
Original release date:
2016-01-06
Authors:
Burmann, Bjoern; Hiller, Sebastian
Citation:

Citation: Stanger, Frederic; Burmann, Bjoern; Harms, Alexander; Correia, Hugo; Mazur, Adam; Sharpe, Timothy; Dehio, Christoph; Hiller, Sebastian; Schirmer, Tilman. "Intrinsic regulation of FIC-domain AMP-transferases by oligomerization and automodification"  Proc. Natl. Acad. Sci. U. S. A. ., .-..

Assembly members:

Assembly members:
NmFic, polymer, 188 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: b-proteobacteria   Taxonomy ID: 487   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Neisseria meningitidis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSF-DUET1

Data sets:
Data typeCount
13C chemical shifts524
15N chemical shifts172
1H chemical shifts172

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NmFic1

Entities:

Entity 1, NmFic 188 residues - Formula weight is not available

1   METHISHISHISHISHISHISMETLYSSER
2   ILEASPGLUGLNSERLEUHISASNALAARG
3   ARGLEUPHEGLUSERGLYASPILEASPARG
4   ILEGLUVALGLYTHRTHRALAGLYLEUGLN
5   GLNILEHISARGTYRLEUPHEGLYGLYLEU
6   TYRASPPHEALAGLYGLNILEARGGLUASP
7   ASNILESERLYSGLYGLYPHEARGPHEALA
8   ASNALAMETTYRLEULYSGLUALALEUVAL
9   LYSILEGLUGLNMETPROGLUARGTHRPHE
10   GLUGLUILEILEALALYSTYRVALARGMET
11   ASNILEALAALAPROPHELEUGLUGLYASN
12   GLYARGSERTHRARGILETRPLEUASPLEU
13   VALLEULYSLYSASNLEULYSLYSVALVAL
14   ASNTRPGLNASNVALSERLYSTHRLEUTYR
15   LEUGLNALAMETGLUARGSERPROVALASN
16   ASPLEUARGLEUARGPHELEULEULYSASP
17   ASNLEUTHRASPASPVALASPASNARGGLU
18   ILEILEPHELYSGLYILEGLUGLNSERTYR
19   TYRTYRGLUGLYTYRGLULYSGLY

Samples:

sample_1: NmFic, [U-100% 13C; U-100% 15N], 500 uM; D2O 10%; MES 25 mM; sodium chloride 150 mM; H2O 90%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin, Guntert, Keller and Wuthrich - chemical shift assignment, collection, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks