BMRB Entry 25750

Title:
NMR Structure of the C-terminal Coiled-Coil Domain of CIN85
Deposition date:
2015-08-11
Original release date:
2016-07-11
Authors:
Habeck, Michael; Becker, Stefan; Griesinger, Christian; Wong, Leo
Citation:

Citation: Kuehn, Julius; Wong, Leo; Pirkuliyeva, Sona; Batista, Facundo; Schwiegk, Claudia; Gencalp, Kevser; Urlaub, Henning; Habeck, Michael; Becker, Stefan; Griesinger, Christian; Wienands, Juergen. "The adaptor protein CIN85 assembles intracellular signaling clusters for B cell activation"  Sci. Signal. 9, 66-66 (2016).
PubMed: 27353366

Assembly members:

Assembly members:
CIN85_CC-domain, polymer, 75 residues, 8762.257 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts277
15N chemical shifts79
1H chemical shifts301
T2 relaxation values60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CIN85 CC-domain, chain 11
2CIN85 CC-domain, chain 21
3CIN85 CC-domain, chain 31

Entities:

Entity 1, CIN85 CC-domain, chain 1 75 residues - 8762.257 Da.

1   GLYHISMETGLUGLYLYSPROLYSMETGLU
2   PROALAALASERSERGLNALAALAVALGLU
3   GLULEUARGTHRGLNVALARGGLULEUARG
4   SERILEILEGLUTHRMETLYSASPGLNGLN
5   LYSARGGLUILELYSGLNLEULEUSERGLU
6   LEUASPGLUGLULYSLYSILEARGLEUARG
7   LEUGLNMETGLUVALASNASPILELYSLYS
8   ALALEUGLNSERLYS

Samples:

sample_1: CIN85 CC-domain, [U-13C; U-15N; U-2H], 2 mM

sample_2: CIN85 CC-domain, [U-13C; U-15N; U-2H], 2 mM; CIN85 CC-domain, [U-2H], 2 mM

sample_conditions: pH: 6.9; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY-HSQCsample_1isotropicsample_conditions
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions
3D HNCACBsample_1isotropicsample_conditions
3D HNCOsample_1isotropicsample_conditions
3D HN(CA)COsample_1isotropicsample_conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions
2D TRACTsample_1isotropicsample_conditions

Software:

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP Q96B97
AlphaFold Q9NYR0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks