BMRB Entry 25333

Title:
BILE SALT RECOGNITION BY HUMAN LIVER FATTY ACID BINDING PROTEIN
Deposition date:
2014-11-12
Original release date:
2015-08-25
Authors:
Favretto, Filippo; Santambrogio, Carlo; D'Onofrio, Mariapina; Molinari, Henriette; Grandori, Rita; Assfalg, Michael
Citation:

Citation: Favretto, Filippo; Santambrogio, Carlo; D'Onofrio, Mariapina; Molinari, Henriette; Grandori, Rita; Assfalg, Michael. "Bile salt recognition by human liver fatty acid binding protein"  FEBS J. 282, 1271-1288 (2015).
PubMed: 25639618

Assembly members:

Assembly members:
hL-FABP, polymer, 135 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE50

Data sets:
Data typeCount
13C chemical shifts250
15N chemical shifts130
1H chemical shifts130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human liver fatty acid binding protein1

Entities:

Entity 1, human liver fatty acid binding protein 135 residues - Formula weight is not available

First and last four residues are added for cloning and tag purposes. Variant T94A

1   METARGGLYSERMETSERPHESERGLYLYS
2   TYRGLNLEUGLNSERGLNGLUASNPHEGLU
3   ALAPHEMETLYSALAILEGLYLEUPROGLU
4   GLULEUILEGLNLYSGLYLYSASPILELYS
5   GLYVALSERGLUILEVALGLNASNGLYLYS
6   HISPHELYSPHETHRILETHRALAGLYSER
7   LYSVALILEGLNASNGLUPHETHRVALGLY
8   GLUGLUCYSGLULEUGLUTHRMETTHRGLY
9   GLULYSVALLYSTHRVALVALGLNLEUGLU
10   GLYASPASNLYSLEUVALTHRALAPHELYS
11   ASNILELYSSERVALTHRGLULEUASNGLY
12   ASPILEILETHRASNTHRMETTHRLEUGLY
13   ASPILEVALPHELYSARGILESERLYSARG
14   ILELEUVALPROARG

Samples:

sample_1: human liver fatty acid binding protein, [U-100% 13C; U-100% 15N], 0.5 mM; TAUROCHOLIC ACID, natural abundant, 7.5 mM; sodium phosphate, natural abundant, 10 mM; H2O, natural abundant, 93%; D2O, [U-2H], 7%

sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17302 17303 19160 19188 19189
PDB
DBJ BAI46102
EMBL CAG46887
GB AAA52418 AAA52419 AAH22287 AAH32801 AAX37108
REF NP_001434 XP_001140263 XP_003268834 XP_003805902 XP_004029642
SP P07148
AlphaFold P07148

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks