BMRB Entry 25224

Title:
Backbone 1H, 13C, and 15N resonance assignments of the Fc fragment of human immunoglobulin G glycoprotein
Deposition date:
2014-09-15
Original release date:
2014-10-16
Authors:
Yagi, Hirokazu; Zhang, Ying; Yagi-Utsumi, Maho; Yamaguchi, Takumi; Iida, Shigeru; Yamaguchi, Yoshiki; Kato, Koichi
Citation:

Citation: Yagi, Hirokazu; Zhang, Ying; Yagi-Utsumi, Maho; Yamaguchi, Takumi; Iida, Shigeru; Kato, Koichi. "Backbone 1H, 13C, and 15N resonance assignments of the Fc fragment of human immunoglobulin G glycoprotein"  Biomol. NMR Assignments 9, 257-260 (2015).
PubMed: 25291979

Assembly members:

Assembly members:
human_IgG1-Fc, polymer, 222 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: CHO cell   Vector: not applicable

Data sets:
Data typeCount
13C chemical shifts542
15N chemical shifts198
1H chemical shifts197

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human IgG-Fc, chain 11
2human IgG-Fc, chain 21

Entities:

Entity 1, human IgG-Fc, chain 1 222 residues - Formula weight is not available

1   THRCYSPROPROCYSPROALAPROGLULEU
2   LEUGLYGLYPROSERVALPHELEUPHEPRO
3   PROLYSPROLYSASPTHRLEUMETILESER
4   ARGTHRPROGLUVALTHRCYSVALVALVAL
5   ASPVALSERHISGLUASPPROGLUVALLYS
6   PHEASNTRPTYRVALASPGLYVALGLUVAL
7   HISASNALALYSTHRLYSPROARGGLUGLU
8   GLNTYRASNSERTHRTYRARGVALVALSER
9   VALLEUTHRVALLEUHISGLNASPTRPLEU
10   ASNGLYLYSGLUTYRLYSCYSLYSVALSER
11   ASNLYSALALEUPROALAPROILEGLULYS
12   THRILESERLYSALALYSGLYGLNPROARG
13   GLUPROGLNVALTYRTHRLEUPROPROSER
14   ARGASPGLULEUTHRLYSASNGLNVALSER
15   LEUTHRCYSLEUVALLYSGLYPHETYRPRO
16   SERASPILEALAVALGLUTRPGLUSERASN
17   GLYGLNPROGLUASNASNTYRLYSTHRTHR
18   PROPROVALLEUASPSERASPGLYSERPHE
19   PHELEUTYRSERLYSLEUTHRVALASPLYS
20   SERARGTRPGLNGLNGLYASNVALPHESER
21   CYSSERVALMETHISGLUALALEUHISASN
22   HISTYRTHRGLNLYSSERLEUSERLEUSER
23   PROGLY

Samples:

sample_1: human IgG1-Fc, [U-100% 13C; U-100% 15N], 10 mg/mL; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.0; temperature: 325 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CcpNmr vv2.4.0, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker Avance 800 MHz
  • JEOL ECA 920 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks