BMRB Entry 25220

Title:
N6-Methyladenosine RNA
Deposition date:
2014-09-12
Original release date:
2015-09-04
Authors:
Lynch, Stephen; Kool, Eric
Citation:

Citation: Roost, Caroline; Lynch, Stephen; Batista, Pedro; Qu, Kun; Chang, Howard; Kool, Eric. "Structure and thermodynamics of N6-methyladenosine in RNA: a spring-loaded base modification"  J. Am. Chem. Soc. 137, 2107-2115 (2015).
PubMed: 25611135

Assembly members:

Assembly members:
N-6_Methyl_Adenosine_RNA, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-6_Methyl_Adenosine_RNA: GGXCUAGUCC

Data sets:
Data typeCount
13C chemical shifts28
1H chemical shifts83
31P chemical shifts9

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA duplex, chain 11
2RNA duplex, chain 21

Entities:

Entity 1, RNA duplex, chain 1 10 residues - Formula weight is not available

Residue 3 is methylated on N6 position

1   GG6MDCUAGUCC

Samples:

sample_1: N-6 Methyl Adenosine RNA 0.2 mM; sodium chloride 100 mM; EDTA 0.1 mM; sodium phosphate 10 mM

sample_2: N-6 Methyl Adenosine RNA 0.2 mM; sodium chloride 100 mM; EDTA 0.1 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 100 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-31P COSYsample_2isotropicsample_conditions_1

Software:

VNMR v6.1C, Varian - collection, processing

SPARKY v3.115, Goddard - chemical shift assignment, data analysis

X-PLOR_NIH v2.36, Brunger - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz