BMRB Entry 19818

Title:
Murine Neuroglobin, Fe3+ form (metNgB)
Deposition date:
2014-02-26
Original release date:
2015-03-05
Authors:
Yang, Yin Shan; Allemand, Frederic; Guca, Ewelina; Vallone, Beatrice; Delbecq, Stephane; Roumestand, Christian
Citation:

Citation: Yang, Yin Shan; Allemand, Frederic; Guca, Ewelina; Vallone, Beatrice; Delbecq, Stephane; Roumestand, Christian. "1H, 15N and 13C Backbone resonance assignments of murine met-Neuroglobin, free and in complex with cyanide."  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
metNgb, polymer, 151 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b-tev

Data sets:
Data typeCount
13C chemical shifts853
15N chemical shifts280
1H chemical shifts598

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1metNgb1

Entities:

Entity 1, metNgb 151 residues - Formula weight is not available

1   METGLUARGPROGLUSERGLULEUILEARG
2   GLNSERTRPARGVALVALSERARGSERPRO
3   LEUGLUHISGLYTHRVALLEUPHEALAARG
4   LEUPHEALALEUGLUPROSERLEULEUPRO
5   LEUPHEGLNTYRASNGLYARGGLNPHESER
6   SERPROGLUASPSERLEUSERSERPROGLU
7   PHELEUASPHISILEARGLYSVALMETLEU
8   VALILEASPALAALAVALTHRASNVALGLU
9   ASPLEUSERSERLEUGLUGLUTYRLEUTHR
10   SERLEUGLYARGLYSHISARGALAVALGLY
11   VALARGLEUSERSERPHESERTHRVALGLY
12   GLUSERLEULEUTYRMETLEUGLULYSSER
13   LEUGLYPROASPPHETHRPROALATHRARG
14   THRALATRPSERARGLEUTYRGLYALAVAL
15   VALGLNALAMETSERARGGLYTRPASPGLY
16   GLU

Samples:

sample_1: metNgb, [U-100% 13C; U-100% 15N], 0.5 mM; Tris, HCl 50 mM; D2O 10%; H2O 90%; PMSF 0.05 mM; DSS 0.05 mM

sample_conditions_1: pH: 7.0; pressure: ambient atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks