BMRB Entry 19721

Title:
NMR backbone assignment of Y75A apo-HasAp from Pseudomonas aeruginosa
Deposition date:
2014-01-07
Original release date:
2014-04-11
Authors:
Yao, Huili; Rivera, Mario
Citation:

Citation: Kumar, Ritesh; Matsumura, Hirotoshi; Lovell, Scott; Yao, Huili; Rodriguez, Juan; Battaile, Kevin; Moenne-Loccoz, Pierre; Rivera, Mario. "Replacing the Axial Ligand Tyrosine 75 or Its Hydrogen Bond Partner Histidine 83 Minimally Affects Hemin Acquisition by the Hemophore HasAp from Pseudomonas aeruginosa."  Biochemistry 53, 2112-2125 (2014).
PubMed: 24625274

Assembly members:

Assembly members:
Y75A_apo-HasAp, polymer, 184 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 11a

Data sets:
Data typeCount
13C chemical shifts164
15N chemical shifts149
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Y75A apo HasAp1

Entities:

Entity 1, Y75A apo HasAp 184 residues - Formula weight is not available

1   METSERILESERILESERTYRSERTHRTHR
2   TYRSERGLYTRPTHRVALALAASPTYRLEU
3   ALAASPTRPSERALATYRPHEGLYASPVAL
4   ASNHISARGPROGLYGLNVALVALASPGLY
5   SERASNTHRGLYGLYPHEASNPROGLYPRO
6   PHEASPGLYSERGLNTYRALALEULYSSER
7   THRALASERASPALAALAPHEILEALAGLY
8   GLYASPLEUHISALATHRLEUPHESERASN
9   PROSERHISTHRLEUTRPGLYLYSLEUASP
10   SERILEALALEUGLYASPTHRLEUTHRGLY
11   GLYALASERSERGLYGLYTYRALALEUASP
12   SERGLNGLUVALSERPHESERASNLEUGLY
13   LEUASPSERPROILEALAGLNGLYARGASP
14   GLYTHRVALHISLYSVALVALTYRGLYLEU
15   METSERGLYASPSERSERALALEUGLNGLY
16   GLNILEASPALALEULEULYSALAVALASP
17   PROSERLEUSERILEASNSERTHRPHEASP
18   GLNLEUALAALAALAGLYVALALAHISALA
19   THRPROALAALA

Samples:

sample_1: Y75A apo-HasAp, [U-99% 13C; U-99% 15N], 2.5 mM; H2O 95%; D2O 5%; HPO4- 20 mM

sample_conditions_1: ionic strength: 0.12 M; pH: 7.0; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15962 15963 19720 19722
PDB
DBJ BAK91367 BAP21966 BAP49596 BAQ38483 BAR66548
EMBL CAA76520 CAW26382 CCQ88896 CDH69922 CDH76002
GB AAG06795 AAT49927 ABJ12662 ABR84528 AEO74046
REF NP_252097 WP_003091842 WP_003115011 WP_003124283 WP_003153113

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks