BMRB Entry 19607

Title:
The C-terminal domain of SRA1p has a fold more similar to PRP18 than to an RRM and does not directly bind to the SRA1 RNA STR7 region.
Deposition date:
2013-11-10
Original release date:
2014-02-10
Authors:
Bilinovich, Stephanie; Davis, Caroline; Morris, Daniel; Ray, Louis; Prokop, Jeremy; Buchan, Greg; Leeper, Thomas
Citation:

Citation: Bilinovich, Stephanie; Davis, Caroline; Morris, Daniel; Ray, Louis; Prokop, Jeremy; Buchan, Gregory; Leeper, Thomas. "The C-Terminal Domain of SRA1p Has a Fold More Similar to PRP18 than to an RRM and Does Not Directly Bind to the SRA1 RNA STR7 Region."  J. Mol. Biol. ., .-. (2014).
PubMed: 24486611

Assembly members:

Assembly members:
SRA1p_C-terminal_domain, polymer, 131 residues, 14769.870 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJexpress

Data sets:
Data typeCount
13C chemical shifts536
15N chemical shifts130
1H chemical shifts789

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal domain of SRA1p1

Entities:

Entity 1, C-terminal domain of SRA1p 131 residues - 14769.870 Da.

1   GLYSERGLUALAVALMETGLUASPVALLEU
2   ARGPROLEUGLUGLNALALEUGLUASPCYS
3   ARGGLYHISTHRARGLYSGLNVALCYSASP
4   ASPILESERARGARGLEUALALEULEUGLN
5   GLUGLNTRPALAGLYGLYLYSLEUSERILE
6   PROVALLYSLYSARGMETALALEULEUVAL
7   GLNGLULEUSERSERHISARGTRPASPALA
8   ALAASPASPILEHISARGSERLEUMETVAL
9   ASPHISVALTHRGLUVALSERGLNTRPMET
10   VALGLYVALLYSARGLEUILEALAGLULYS
11   ARGSERLEUPHESERGLUGLUALAALAASN
12   GLUGLULYSSERALAALATHRALAGLULYS
13   ASNHISTHRILEPROGLYPHEGLNGLNALA
14   SER

Samples:

sample_1: sodium phosphate 50 mM; DTT 2 mM; sodium chloride 50 mM; SRA1p C-terminal domain, [U-98% 13C; U-98% 15N], 0.6 mM; H2O 95%; D2O 5%

sample_2: sodium phosphate 50 mM; DTT 2 mM; sodium chloride 50 mM; SRA1p C-terminal domain, [U-98% 13C; U-98% 15N], 0.6 mM; D2O 100%

sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HN(COCA)CBsample_1anisotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3d HNCAintrasample_1isotropicsample_conditions_1
3D HNCACBintrasample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D HBCBCGCDCDHEsample_1isotropicsample_conditions_1
2D HBCBCGCDHDsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

ARIA v2.3, Linge, O'Donoghue and Nilges - refinement, structure solution

CCPN, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

UNP Q9HD15.1
PDB
DBJ BAG51445 BAJ20657
EMBL CAH93286
GB AAG02114 AAG02115 AAG02116 AAH40043 AAI52789
REF NP_001030312 NP_001240693 XP_001086694 XP_001137717 XP_002816012
SP Q9HD15
AlphaFold Q9HD14 Q9HD15

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks