BMRB Entry 19496

Title:
HN, NH, CA, CB and methyl group assignments of Filamin A three Ig-domain fragment FLNa3-5
Deposition date:
2013-09-16
Original release date:
2014-02-12
Authors:
Tossavainen, Helena; Permi, Perttu
Citation:

Citation: Tossavainen, Helena; Seppala, Jonne; Sethi, Ritika; Pihlajamaa, Tero; Permi, Perttu. "H(N), N(H), C (), C (), and methyl group assignments of filamin multidomain fragments IgFLNc4-5 and IgFLNa3-5."  Biomol. NMR Assignments ., .-. (2014).
PubMed: 24414222

Assembly members:

Assembly members:
FLNa3-5, polymer, 291 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGTVL1

Data sets:
Data typeCount
13C chemical shifts670
15N chemical shifts258
1H chemical shifts678

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FLNa3-51

Entities:

Entity 1, FLNa3-5 291 residues - Formula weight is not available

1   SERMETCYSASNPROSERALACYSARGALA
2   VALGLYARGGLYLEUGLNPROLYSGLYVAL
3   ARGVALLYSGLUTHRALAASPPHELYSVAL
4   TYRTHRLYSGLYALAGLYSERGLYGLULEU
5   LYSVALTHRVALLYSGLYPROLYSGLYGLU
6   GLUARGVALLYSGLNLYSASPLEUGLYASP
7   GLYVALTYRGLYPHEGLUTYRTYRPROMET
8   VALPROGLYTHRTYRILEVALTHRILETHR
9   TRPGLYGLYGLNASNILEGLYARGSERPRO
10   PHEGLUVALLYSVALGLYTHRGLUCYSGLY
11   ASNGLNLYSVALARGALATRPGLYPROGLY
12   LEUGLUGLYGLYVALVALGLYLYSSERALA
13   ASPPHEVALVALGLUALAILEGLYASPASP
14   VALGLYTHRLEUGLYPHESERVALGLUGLY
15   PROSERGLNALALYSILEGLUCYSASPASP
16   LYSGLYASPGLYSERCYSASPVALARGTYR
17   TRPPROGLNGLUALAGLYGLUTYRALAVAL
18   HISVALLEUCYSASNSERGLUASPILEARG
19   LEUSERPROPHEMETALAASPILEARGASP
20   ALAPROGLNASPPHEHISPROASPARGVAL
21   LYSALAARGGLYPROGLYLEUGLULYSTHR
22   GLYVALALAVALASNLYSPROALAGLUPHE
23   THRVALASPALALYSHISGLYGLYLYSALA
24   PROLEUARGVALGLNVALGLNASPASNGLU
25   GLYCYSPROVALGLUALALEUVALLYSASP
26   ASNGLYASNGLYTHRTYRSERCYSSERTYR
27   VALPROARGLYSPROVALLYSHISTHRALA
28   METVALSERTRPGLYGLYVALSERILEPRO
29   ASNSERPROPHEARGVALASNVALGLYALA
30   GLY

Samples:

sample_1: FLNa3-5, [U-13C; U-15N], 0.9 mM; sodium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
HCCmHm-TOCSYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Agilent - collection

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks