BMRB Entry 19495

Name: HN, NH, CA, CB and methyl group assignments of Filamin C two Ig-domain fragment FLNc4-5
Published: 2014-02-12

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19495

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HN, NH, CA, CB and methyl group assignments of Filamin C two Ig-domain fragment FLNc4-5

Deposition date:

2013-09-16

Original release date:

2014-02-12

Authors:

Tossavainen, Helena; Permi, Perttu

Citation:

Citation: Tossavainen, Helena; Seppala, Jonne; Sethi, Ritika; Pihlajamaa, Tero; Permi, Perttu. "H(N), N(H), C (), C (), and methyl group assignments of filamin multidomain fragments IgFLNc4-5 and IgFLNa3-5." Biomol. NMR Assignments ., .-. (2014).
PubMed: 24414222

Assembly members:

Assembly members:
FLNc4-5, polymer, 195 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGTVL1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FLNc4-5: SMAGVQKVRAWGPGLETGQV GKSADFVVEAIGTEVGTLGF SIEGPSQAKIECDDKGDGSC DVRYWPTEPGEYAVHVICDD EDIRDSPFIAHILPAPPDCF PDKVKAFGPGLEPTGCIVDK PAEFTIDARAAGKGDLKLYA QDADGCPIDIKVIPNGDGTF RCSYVPTKPIKHTIIISWGG VNVPKSPFRVNVGEG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	495
15N chemical shifts	169
1H chemical shifts	457

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FLNc4-5	1

Entities:

Entity 1, FLNc4-5 195 residues - Formula weight is not available

1

SER

MET

ALA

GLY

VAL

GLN

LYS

VAL

ARG

ALA

2

TRP

GLY

PRO

GLY

LEU

GLU

THR

GLY

GLN

VAL

3

GLY

LYS

SER

ALA

ASP

PHE

VAL

GLU

ALA

4

ILE

GLY

THR

GLU

VAL

GLY

THR

LEU

GLY

PHE

5

SER

ILE

GLU

GLY

PRO

SER

GLN

ALA

LYS

ILE

6

GLU

CYS

ASP

LYS

GLY

ASP

GLY

SER

CYS

7

ASP

VAL

ARG

TYR

TRP

PRO

THR

GLU

PRO

GLY

8

GLU

TYR

ALA

VAL

HIS

VAL

ILE

CYS

ASP

9

GLU

ASP

ILE

ARG

ASP

SER

PRO

PHE

ILE

ALA

10

HIS

ILE

LEU

PRO

ALA

PRO

ASP

CYS

PHE

11

PRO

ASP

LYS

VAL

LYS

ALA

PHE

GLY

PRO

GLY

12

LEU

GLU

PRO

THR

GLY

CYS

ILE

VAL

ASP

LYS

13

PRO

ALA

GLU

PHE

THR

ILE

ASP

ALA

ARG

ALA

14

ALA

GLY

LYS

GLY

ASP

LEU

LYS

LEU

TYR

ALA

15

GLN

ASP

ALA

ASP

GLY

CYS

PRO

ILE

ASP

ILE

16

LYS

VAL

ILE

PRO

ASN

GLY

ASP

GLY

THR

PHE

17

ARG

CYS

SER

TYR

VAL

PRO

THR

LYS

PRO

ILE

18

LYS

HIS

THR

ILE

SER

TRP

GLY

19

VAL

ASN

VAL

PRO

LYS

SER

PRO

PHE

ARG

VAL

20

ASN

VAL

GLY

GLU

GLY

Samples:

sample_1: FLNc4-5, [U-13C; U-15N], 0.9 mM; sodium phosphate 50 mM; sodium chloride 100 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
HCCmHm-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ, Agilent - collection

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 800 MHz