BMRB Entry 19321

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19321

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Title:
1H,15N,13C resonance assignments of cerato populin, a fungal PAMP from Ceratocystis populicola
Deposition date:
2013-06-26
Original release date:
2013-09-18
Authors:
Baroni, Fabio; Pazzagli, Luigia; Cappugi, Gianni; Scala, Aniello; Martellini, Federica; Fadel, Valmir; Franzoni, Lorella; Spisni, Alberto; Pertinhez, Thelma
Citation:

Citation: Comparini, Cecilia; Carresi, Lara; Pagni, Eleonora; Sbrana, Francesca; Sebastiani, Federico; Luchi, Nicola; Santini, Alberto; Capretti, Paolo; Tiribilli, Bruno; Pazzagli, Luigia; Cappugi, Gianni; Scala, Aniello. "New protein orthologous to cerato-platanin in various Ceratocystis species and the purification and characterization of cerato-populin from Ceratocystis populicola"  Appl. Microbiol. Biotechnol. 84, 309-322 (2009).
PubMed: 19387635

Assembly members:

Assembly members:
cerato_populin, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Ascomycetes   Taxonomy ID: 312341   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Ceratocystis populicola

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPIC9

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cerato_populin: EAEAVSISYDPVYGNPGQSM NTVACSNGKFGLAAKYPTLG DLPAFPNVGGIPDIPDWNND QNCGTCWKVTVPNRNPIFIL GVDSGKGNFNVSPQAFTELT GSTDAGRVNDIQYEKVSADN CKMPVA

Data sets:
Data typeCount
13C chemical shifts528
15N chemical shifts135
1H chemical shifts826

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cerato populin1

Entities:

Entity 1, cerato populin 126 residues - Formula weight is not available

1   GLUALAGLUALAVALSERILESERTYRASP
2   PROVALTYRGLYASNPROGLYGLNSERMET
3   ASNTHRVALALACYSSERASNGLYLYSPHE
4   GLYLEUALAALALYSTYRPROTHRLEUGLY
5   ASPLEUPROALAPHEPROASNVALGLYGLY
6   ILEPROASPILEPROASPTRPASNASNASP
7   GLNASNCYSGLYTHRCYSTRPLYSVALTHR
8   VALPROASNARGASNPROILEPHEILELEU
9   GLYVALASPSERGLYLYSGLYASNPHEASN
10   VALSERPROGLNALAPHETHRGLULEUTHR
11   GLYSERTHRASPALAGLYARGVALASNASP
12   ILEGLNTYRGLULYSVALSERALAASPASN
13   CYSLYSMETPROVALALA

Samples:

sample_1: cerato populin, [U-13C; U-15N], 280 uM; H2O 95%; D2O 5%

sample_conditions_1: pH: 3; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQC NH2 onlysample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

GB ABM63506

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks