BMRB Entry 19095

Title:
Backbone and side-chain 1H, 13C and 15N resonance assignments of the OB domain of the single-stranded DNA binding protein from Sulfolobus Solfataricus
Deposition date:
2013-03-17
Original release date:
2013-04-17
Authors:
Gamsjaeger, Roland; Kariawasam, Ruvini; Touma, Christine; White, Malcolm; Cubeddu, Liza
Citation:

Citation: Gamsjaeger, Roland; Kariawasam, Ruvini; Touma, Christine; Kwan, Ann; White, Malcolm; Cubeddu, Liza. "Backbone and side-chain (1)H, (13)C and (15)N resonance assignments of the OB domain of the single stranded DNA binding protein from Sulfolobus solfataricus and chemical shift mapping of the DNA-binding interface."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23749431

Assembly members:

Assembly members:
SsoSSB, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Crenarchaeotes   Taxonomy ID: 2287   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus Solfataricus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28C

Data sets:
Data typeCount
13C chemical shifts505
15N chemical shifts133
1H chemical shifts829

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SsoSSB1

Entities:

Entity 1, SsoSSB 117 residues - Formula weight is not available

1   METGLUGLULYSVALGLYASNLEULYSPRO
2   ASNMETGLUSERVALASNVALTHRVALARG
3   VALLEUGLUALASERGLUALAARGGLNILE
4   GLNTHRLYSASNGLYVALARGTHRILESER
5   GLUALAILEVALGLYASPGLUTHRGLYARG
6   VALLYSLEUTHRLEUTRPGLYLYSHISALA
7   GLYSERILELYSGLUGLYGLNVALVALLYS
8   ILEGLUASNALATRPTHRTHRALAPHELYS
9   GLYGLNVALGLNLEUASNALAGLYSERLYS
10   THRLYSILEALAGLUALASERGLUASPGLY
11   PHEPROGLUSERSERGLNILEPROGLUASN
12   THRPROTHRALAARGARGARG

Samples:

sample_1: SsoSSB, [U-100% 13C; U-100% 15N], 0.8 – 1.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 60 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CC(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D HCC(CO)NH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAK42515 ACP34347 ACP37052 ACP44458 ACP54191
REF WP_009989484 WP_012710339
SP Q97W73
AlphaFold Q97W73

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks