BMRB Entry 19069

Title:
1H,13C,15N Assignement of EFGP fluorescent protein
Deposition date:
2013-03-01
Original release date:
2013-04-02
Authors:
Juranic, Nenad; Atanasova, Elena; Prendergast, Franklyn
Citation:

Citation: Juranic, Nenad; Atanasova, Elena; Prendergast, Franklyn. "NMR signature of HBDI chromophore in folded EFGP"  .

Assembly members:

Assembly members:
EFGP, polymer, 241 residues, 319.313 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Data sets:
Data typeCount
13C chemical shifts1037
15N chemical shifts245
1H chemical shifts1663

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EFGP1

Entities:

Entity 1, EFGP 241 residues - 319.313 Da.

Non-standard residue name of the chromophore CRO(65-67) created from residues T65,Y66,G67

1   GLYHISMETVALSERLYSGLYGLUGLULEU
2   PHETHRGLYVALVALPROILELEUVALGLU
3   LEUASPGLYASPVALASNGLYHISLYSPHE
4   SERVALSERGLYGLUGLYGLUGLYASPALA
5   THRTYRGLYLYSLEUTHRLEULYSPHEILE
6   CYSTHRTHRGLYLYSLEUPROVALPROTRP
7   PROTHRLEUVALTHRTHRLEUCROCROCRO
8   VALGLNCYSPHESERARGTYRPROASPHIS
9   METLYSGLNHISASPPHEPHELYSSERALA
10   METPROGLUGLYTYRVALGLNGLUARGTHR
11   ILEPHEPHELYSASPASPGLYASNTYRLYS
12   THRARGALAGLUVALLYSPHEGLUGLYASP
13   THRLEUVALASNARGILEGLULEULYSGLY
14   ILEASPPHELYSGLUASPGLYASNILELEU
15   GLYHISLYSLEUGLUTYRASNTYRASNSER
16   HISASNVALTYRILEMETALAASPLYSGLN
17   LYSASNGLYILELYSVALASNPHELYSILE
18   ARGHISASNILEGLUASPGLYSERVALGLN
19   LEUALAASPHISTYRGLNGLNASNTHRPRO
20   ILEGLYASPGLYPROVALLEULEUPROASP
21   ASNHISTYRLEUSERTHRGLNSERALALEU
22   SERLYSASPPROASNGLULYSARGASPHIS
23   METVALLEULEUGLUPHEVALTHRALAALA
24   GLYILETHRLEUGLYMETASPGLULEUTYR
25   LYS

Samples:

sample_1: D20 5%; H20 95%; EFGP 0.4 mM; NaH2PO4/Na2HPO4 50 mM; NaCl 300 mM

sample_2: D20 5%; H20 95%; EFGP 0.4 mM; NaH2PO4/Na2HPO4 50 mM; NaCl 300 mM

sample_conditions_1: ionic strength: 350 mM; pH: 7.7; pressure: 1 atm; temperature: 308 K

sample_conditions_2: ionic strength: 350 mM; pH: 7.7; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D C-HCACOsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D C-HCACOsample_2isotropicsample_conditions_1
3D C-HB(CBCA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1anisotropicsample_conditions_2
3D HNCOsample_1anisotropicsample_conditions_2

Software:

FELIX v2007, Accelrys Software Inc. - peak picking, processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAB11884 BAB32740 BAB61869 BAB61870 BAB61871
EMBL CAC40615 CAC40618 CAC40622 CAC40625 CAC40629
GB AAA27721 AAA69675 AAB01173 AAB02572 AAB02574
REF WP_012569506 WP_031943942 YP_002302326 YP_009062989
SP P42212
AlphaFold P42212

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks