BMRB Entry 19067

Title:
Solution structure of latherin
Deposition date:
2013-03-01
Original release date:
2013-06-24
Authors:
Vance, S.; Macdonald, R.; Cooper, A.; Kennedy, M.; Smith, Brian
Citation:

Citation: Vance, S.; Macdonald, R.; Cooper, A.; Kennedy, M.; Smith, Brian. "The structure of latherin, a surfactant allergen protein from horse sweat and saliva"  To Be Published ., .-..

Assembly members:

Assembly members:
LATHERIN, polymer, 211 residues, 22883.3271 Da.

Natural source:

Natural source:   Common Name: Horse   Taxonomy ID: 9796   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32

Data sets:
Data typeCount
13C chemical shifts917
15N chemical shifts205
1H chemical shifts1527

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LATHERIN1

Entities:

Entity 1, LATHERIN 211 residues - 22883.3271 Da.

1   ALAMETALAGLNGLNILEPROPROGLUVAL
2   SERSERGLNILETHRASPALALEUTHRGLN
3   GLYLEULEUASPGLYASNPHELEUSERLEU
4   LEUASNALAILEASNLEUGLUGLYLEULEU
5   ASNTHRILELEUASPGLNVALTHRGLYLEU
6   LEUASNILELEUVALGLYPROLEULEUGLY
7   PROSERASPALAGLUILELYSLEUGLNASP
8   THRARGLEULEUGLNLEUSERLEUGLUPHE
9   SERPROASPSERLYSGLYILEASPILETRP
10   ILEPROLEUGLULEUSERVALTYRLEULYS
11   LEULEUILELEUGLUPROLEUTHRLEUTYR
12   VALARGTHRASPILEARGVALGLNLEUARG
13   LEUGLUSERASPGLUASPGLYLYSTYRARG
14   LEUALAPHEGLYHISCYSSERLEULEUPRO
15   ARGALAILEGLULEUGLNSERGLYASNPRO
16   LEUSERLEUPROVALASNALAVALLEUGLY
17   THRILEGLUASNALALEUGLYASNPHEILE
18   THRGLUASPLEUGLYALAGLYLEUCYSPRO
19   THRLEUASNSERLEUVALSERASNLEUASP
20   LEUGLNLEUVALASNASNLEUILEASNLEU
21   ILELEUASPARGALAASNVALASPLEUSER
22   VAL

Samples:

sample_1: LATHERIN, [U-13C; U-15N], 0.5 mM; sodium chloride 0.05 M; NaPi 0.02 M

sample_conditions_1: ionic strength: 0.070 M; pH: 7.500; pressure: 1.000 atm; temperature: 310.000 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCsample_1solutionsample_conditions_1
CbCa(Co)NHsample_1solutionsample_conditions_1
CcCoNHsample_1solutionsample_conditions_1
HNCaCbsample_1solutionsample_conditions_1
HNCaCosample_1solutionsample_conditions_1
HNCosample_1solutionsample_conditions_1
HbHa(Co)NHsample_1solutionsample_conditions_1
HbHaNHsample_1solutionsample_conditions_1
HccCoNHsample_1solutionsample_conditions_1
BMx C_NOESYsample_1solutionsample_conditions_1
C HSQC Bsample_1solutionsample_conditions_1
CH3_TOCSYsample_1solutionsample_conditions_1
HcCoNH Bsample_1solutionsample_conditions_1
Mx N_NOESYsample_1solutionsample_conditions_1
cHCH3_TOCSYsample_1solutionsample_conditions_1
hCCoNHsample_1solutionsample_conditions_1

Software:

AutoDep v4.3 - chemical shift assignment

CNS v1.2, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- -

ANALYSIS v2.0 -

DANGLE v1.1 -

TOPSPIN v1.3 -

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

GB AAM09530 AAM09530 AAW78663 AAW78664
UNP LATH_HORSE
PDB
REF NP_001075328 XP_008506670
SP P82615
AlphaFold Q8SPI9 P82615

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks