BMRB Entry 18685
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18685
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Citation: Aden, Jorgen; Verma, Abhinav; Schug, Alexander; Wolf-Watz, Magnus. "Modulation of a Pre-existing Conformational Equilibrium Tunes Adenylate Kinase Activity." J. Am. Chem. Soc. 134, 16562-16570 (2012).
PubMed: 22963267
Assembly members:
adenylate_kinase, polymer, 214 residues, Formula weight is not available
entity_AP5, non-polymer, 916.367 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
- H_exch_rates
| Data type | Count |
| H exchange rates | 1 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | adenylate kinase | 1 |
| 2 | Ap5A | 2 |
Entities:
Entity 1, adenylate kinase 214 residues - Formula weight is not available
| 1 | MET | ARG | ILE | ILE | LEU | LEU | GLY | ALA | PRO | GLY | ||||
| 2 | ALA | GLY | LYS | GLY | THR | GLN | ALA | GLN | PHE | ILE | ||||
| 3 | MET | GLU | LYS | TYR | GLY | ILE | PRO | GLN | ILE | SER | ||||
| 4 | THR | GLY | ASP | MET | LEU | ARG | ALA | ALA | VAL | LYS | ||||
| 5 | SER | GLY | SER | GLU | LEU | GLY | LYS | GLN | ALA | LYS | ||||
| 6 | ASP | ILE | MET | ASP | ALA | GLY | LYS | LEU | VAL | THR | ||||
| 7 | ASP | GLU | LEU | VAL | ILE | ALA | LEU | VAL | LYS | GLU | ||||
| 8 | ARG | ILE | ALA | GLN | GLU | ASP | CYS | ARG | ASN | GLY | ||||
| 9 | PHE | LEU | LEU | ASP | GLY | PHE | PRO | ARG | THR | ILE | ||||
| 10 | PRO | GLN | ALA | ASP | ALA | MET | LYS | GLU | ALA | GLY | ||||
| 11 | ILE | ASN | VAL | ASP | TYR | VAL | LEU | GLU | PHE | ASP | ||||
| 12 | VAL | PRO | ASP | GLU | LEU | ILE | VAL | ASP | ARG | ILE | ||||
| 13 | VAL | GLY | ARG | ARG | VAL | HIS | ALA | PRO | SER | GLY | ||||
| 14 | ARG | VAL | TYR | HIS | VAL | LYS | PHE | ASN | PRO | PRO | ||||
| 15 | LYS | VAL | GLU | GLY | LYS | ASP | ASP | VAL | THR | GLY | ||||
| 16 | GLU | GLU | LEU | THR | THR | ARG | LYS | ASP | ASP | GLN | ||||
| 17 | GLU | GLU | THR | VAL | ARG | LYS | ARG | LEU | VAL | GLU | ||||
| 18 | TYR | HIS | GLN | MET | THR | ALA | PRO | LEU | ILE | GLY | ||||
| 19 | TYR | TYR | SER | LYS | GLU | ALA | GLU | ALA | GLY | ASN | ||||
| 20 | THR | LYS | TYR | ALA | LYS | VAL | ASP | GLY | THR | LYS | ||||
| 21 | PRO | VAL | ALA | GLU | VAL | ARG | ALA | ASP | LEU | GLU | ||||
| 22 | LYS | ILE | LEU | GLY |
Entity 2, Ap5A - C20 H29 N10 O22 P5 - 916.367 Da.
| 1 | AP5 |
Samples:
sample_1: adenylate kinase, [U-100% 15N], 1 mM; Ap5A 2.5 mM
sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| BMRB | 18683 18686 18687 19089 19090 19091 19092 19093 25353 25357 25360 25361 25362 4152 4193 4350 |
| PDB | |
| DBJ | BAA14303 BAB33950 BAE76253 BAG76023 BAI23848 |
| EMBL | CAA26840 CAF33430 CAF33431 CAF33432 CAF33433 |
| GB | AAA23461 AAB40228 AAC73576 AAG54823 AAM94352 |
| REF | NP_308554 NP_415007 NP_706367 WP_001220233 WP_001220235 |
| SP | A7ZIN4 A7ZXD2 B1IZC0 B1LJN2 B1XFR1 |
| AlphaFold | A7ZIN4 A7ZXD2 B1IZC0 B1LJN2 B1XFR1 |