BMRB Entry 18376

Title:
Free MTIP(61-204) from Plasmodium falciparum
Deposition date:
2012-04-03
Original release date:
2012-04-16
Authors:
Douse, Christopher
Citation:

Citation: Douse, Christopher; Green, Judith; Salgado, Paula; Simpson, Peter; Thomas, Jemima; Langsley, Gordon; Holder, Anthony; Tate, Edward; Cota, Ernesto. "Regulation of the Plasmodium motor complex: phosphorylation of myosin A tail-interacting protein (MTIP) loosens its grip on MyoA."  J. Biol. Chem. 287, 36968-36977 (2012).
PubMed: 22932904

Assembly members:

Assembly members:
MTIP(61-204), polymer, 154 residues, 17605 Da.

Natural source:

Natural source:   Common Name: malaria parasite P. falciparum   Taxonomy ID: 5833   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium falciparum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Data sets:
Data typeCount
13C chemical shifts422
15N chemical shifts137
1H chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MTIP(61-204)1

Entities:

Entity 1, MTIP(61-204) 154 residues - 17605 Da.

1   ARGGLYSERHISHISHISHISHISHISGLY
2   SERVALALAASPILEGLNGLNLEUGLUGLU
3   LYSVALASPGLUSERASPVALARGILETYR
4   PHEASNGLULYSSERSERGLYGLYLYSILE
5   SERILEASPASNALASERTYRASNALAARG
6   LYSLEUGLYLEUALAPROSERSERILEASP
7   GLULYSLYSILELYSGLULEUTYRGLYASP
8   ASNLEUTHRTYRGLUGLNTYRLEUGLUTYR
9   LEUSERILECYSVALHISASPLYSASPASN
10   VALGLUGLULEUILELYSMETPHEALAHIS
11   PHEASPASNASNCYSTHRGLYTYRLEUTHR
12   LYSSERGLNMETLYSASNILELEUTHRTHR
13   TRPGLYASPALALEUTHRASPGLNGLUALA
14   ILEASPALALEUASNALAPHESERSERGLU
15   ASPASNILEASPTYRLYSLEUPHECYSGLU
16   ASPILELEUGLN

Samples:

sample_1: MTIP(61-204), [U-98% 13C; U-98% 15N], 0.5 mM; MOPS buffer 20 mM; sodium chloride 50 mM; TCEP 1 mM; sodium azide 0.005%; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 18377
PDB
EMBL CDO65736
GB AAN36529 ETW17315 ETW29792 ETW35145 ETW41309
REF XP_001350849 XP_012764323

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks