BMRB Entry 18294

Title:
Backbone 1HN, 13C, and 15N Chemical Shift Assignments for Granulocyte colony-stimulating factor A30G
Deposition date:
2012-02-24
Original release date:
2012-08-21
Authors:
Sauve, Simon; Hodgson, Derek; Aubin, Yves
Citation:

Citation: Hodgson, Derek; Thach, William; Gingras, Genevieve; Sauve, Simon; Bristow, Adrian; Aubin, Yves. "Comparability Study of the Structure of Filgrastim and Mutants using the NMR Fingerprint Assay"  J. Pharm. Sci. ., .-..

Assembly members:

Assembly members:
metGCSFA31G, polymer, 175 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b10

Data sets:
Data typeCount
13C chemical shifts475
15N chemical shifts155
1H chemical shifts161

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Granulocyte colony-stimulating factor A30G1

Entities:

Entity 1, Granulocyte colony-stimulating factor A30G 175 residues - Formula weight is not available

Residue 1 is a methionine Residue 31 is mutated from Ala to Gly

1   METTHRPROLEUGLYPROALASERSERLEU
2   PROGLNSERPHELEULEULYSCYSLEUGLU
3   GLNVALARGLYSILEGLNGLYASPGLYALA
4   GLYLEUGLNGLULYSLEUCYSALATHRTYR
5   LYSLEUCYSHISPROGLUGLULEUVALLEU
6   LEUGLYHISSERLEUGLYILEPROTRPALA
7   PROLEUSERSERCYSPROSERGLNALALEU
8   GLNLEUALAGLYCYSLEUSERGLNLEUHIS
9   SERGLYLEUPHELEUTYRGLNGLYLEULEU
10   GLNALALEUGLUGLYILESERPROGLULEU
11   GLYPROTHRLEUASPTHRLEUGLNLEUASP
12   VALALAASPPHEALATHRTHRILETRPGLN
13   GLNMETGLUGLULEUGLYMETALAPROALA
14   LEUGLNPROTHRGLNGLYALAMETPROALA
15   PHEALASERALAPHEGLNARGARGALAGLY
16   GLYVALLEUVALALASERHISLEUGLNSER
17   PHELEUGLUVALSERTYRARGVALLEUARG
18   HISLEUALAGLNPRO

Samples:

sample_1: metGCSFA31G, [U-99% 13C; U-99% 15N], 1 mM; sodium acetate 25 mM; D2O 5%; H2O 95%

sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMRView v8.2.15, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance III 600 MHz

Related Database Links:

BMRB 18291 18293 18295
PDB
DBJ BAF82936 BAG36289 BAJ21174
EMBL CAA27168 CAA27290 CAA27291 CAG46689
GB AAA03056 AAA35882 AAH33245 AAK62469 AAV38741
PRF 1203374A
REF NP_000750 NP_757373 XP_002827665 XP_003278307 XP_003831483
SP P09919
AlphaFold P09919

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks