BMRB Entry 18099

Title:
Solution structure of Ca2+-CIB1 in complex with the cytoplasmic domain of the integrin aIIb subunit
Deposition date:
2011-11-22
Original release date:
2012-02-01
Authors:
Huang, Hao; Vogel, Hans
Citation:

Citation: Huang, Hao; Vogel, Hans. "Structural basis for the activation of platelet integrin IIb3 by calcium- and integrin-binding protein 1."  J. Am. Chem. Soc. 134, 3864-3872 (2012).
PubMed: 22283712

Assembly members:

Assembly members:
CIB1, polymer, 214 residues, 21093.814 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-19b

Data sets:
Data typeCount
13C chemical shifts591
15N chemical shifts159
1H chemical shifts369

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CIB11
2CALCIUM ION_12
3CALCIUM ION_22

Entities:

Entity 1, CIB1 214 residues - 21093.814 Da.

1   METGLYHISHISHISHISHISHISHISHIS
2   HISHISSERSERGLYHISILEASPASPASP
3   ASPLYSHISMETGLYGLYSERGLYSERARG
4   LEUSERLYSGLULEULEUALAGLUTYRGLN
5   ASPLEUTHRPHELEUTHRLYSGLNGLUILE
6   LEULEUALAHISARGARGPHECYSGLULEU
7   LEUPROGLNGLUGLNARGSERVALGLUSER
8   SERLEUARGALAGLNVALPROPHEGLUGLN
9   ILELEUSERLEUPROGLULEULYSALAASN
10   PROPHELYSGLUARGILECYSARGVALPHE
11   SERTHRSERPROALALYSASPSERLEUSER
12   PHEGLUASPPHELEUASPLEULEUSERVAL
13   PHESERASPTHRALATHRPROASPILELYS
14   SERHISTYRALAPHEARGILEPHEASPPHE
15   ASPASPASPGLYTHRLEUASNARGGLUASP
16   LEUSERARGLEUVALASNCYSLEUTHRGLY
17   GLUGLYGLUASPTHRARGLEUSERALASER
18   GLUMETLYSGLNLEUILEASPASNILELEU
19   GLUGLUSERASPILEASPARGASPGLYTHR
20   ILEASNLEUSERGLUPHEGLNHISVALILE
21   SERARGSERPROASPPHEALASERSERPHE
22   LYSILEVALLEU

Entity 2, CALCIUM ION_1 - Ca - 40.078 Da.

1   CA

Samples:

backboneAssignment: CIB1, [U-13C; U-15N; U-2H], 0.5 mM; DTT, [U-2H], 5 mM; CALCIUM ION 2 mM; sodium chloride 100 mM; HEPES 50 mM; aIIb peptide 0.6 mM; H2O 90%; D2O 10%

MethylAssignment: CIB1, [U-13C; U-2H], I/L/V methyl [1H,13C], 0.5 mM; DTT, [U-2H], 5 mM; CALCIUM ION 2 mM; sodium chloride 100 mM; HEPES 50 mM; aIIb peptide 0.6 mM; D2O 100%

MethylSteroAssignment: CIB1, [U-10% 13C], 0.5 mM; DTT, [U-2H], 5 mM; CALCIUM ION 2 mM; sodium chloride 100 mM; HEPES 50 mM; aIIb peptide 0.6 mM; D2O 100%

methylCrossSat: CIB1, [U-2H], I/L/V methyl [1H,13C], 0.5 mM; DTT, [U-2H], 5 mM; CALCIUM ION 2 mM; sodium chloride 100 mM; HEPES 50 mM; aIIb peptide 0.6 mM; D2O 100%

backboneRDC_pf1: CIB1, [U-13C; U-15N; U-2H], 0.5 mM; DTT, [U-2H], 5 mM; CALCIUM ION 2 mM; sodium chloride 100 mM; HEPES 50 mM; aIIb peptide 0.6 mM; pf1 phage 14 mg/mL; H2O 90%; D2O 10%

backboneRDC_PEG: CIB1, [U-13C; U-15N; U-2H], 0.5 mM; DTT, [U-2H], 5 mM; CALCIUM ION 2 mM; sodium chloride 100 mM; HEPES 50 mM; aIIb peptide 0.6 mM; C12E5/glycerol (0.96/1) 5%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.2 M; pH: 7.5; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBbackboneAssignmentisotropicsample_conditions_1
3D HNCObackboneAssignmentisotropicsample_conditions_1
3D HCACObackboneAssignmentisotropicsample_conditions_1
3D HN(COCA)CBbackboneAssignmentisotropicsample_conditions_1
3D HNCO type RDCbackboneRDC_pf1anisotropicsample_conditions_1
2D 1H-15N HSQC IPAPbackboneRDC_pf1anisotropicsample_conditions_1
3D HCCH-TOCSYMethylAssignmentisotropicsample_conditions_1
2D 1H-13C HSQCMethylSteroAssignmentisotropicsample_conditions_1
3D HNCO type RDCbackboneRDC_PEGanisotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 17328 17329
PDB
DBJ BAA36281 BAG70090 BAG70220 BAG72967
EMBL CAG33236
GB AAB39758 AAB53387 AAC51106 AAH00846 ABM82122
REF NP_006375 XP_001096008 XP_003268547 XP_003807774 XP_003901434
SP Q99828
AlphaFold Q99828

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks