BMRB Entry 17960

Title:
Complete 1H, 13C and 15N assignments of the kinetochore localisation domain of BUBR1 bound to a Blinkin motif
Deposition date:
2011-09-26
Original release date:
2011-11-21
Authors:
Simpson, Peter; Blundell, Tom; Cota-Segura, Ernesto; Bolanos-Garcia, Victor
Citation:

Citation: Bolanos-Garcia, Victor; Lischetti, Tiziana; Matak-Vinkovic, Dijana; Cota, Ernesto; Simpson, Pete; Chirgadze, Dimitri; Spring, David; Robinson, Carol; Nilsson, Jakob; Blundell, Tom. "Structure of a Blinkin-BUBR1 complex reveals an interaction crucial for kinetochore-mitotic checkpoint regulation via an unanticipated binding Site."  Structure 19, 1691-1700 (2011).
PubMed: 22000412

Assembly members:

Assembly members:
Bubr1N, polymer, 167 residues, 19844.2 Da.
Blinkin_peptide, polymer, 15 residues, 1826.2 Da.

Natural source:

Natural source:   Common Name: humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Data sets:
Data typeCount
13C chemical shifts715
1H chemical shifts1043
15N chemical shifts162

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bubr1 N domain1
2Blinkin residues 212-2262

Entities:

Entity 1, Bubr1 N domain 167 residues - 19844.2 Da.

The first 3 residues are vector encoded. Q4 corresponds to Q57 of the native sequence

1   GLYPROLEUGLNGLNLYSARGALAPHEGLU
2   TYRGLUILEARGPHETYRTHRGLYASNASP
3   PROLEUASPVALTRPASPARGTYRILESER
4   TRPTHRGLUGLNASNTYRPROGLNGLYGLY
5   LYSGLUSERASNMETSERTHRLEULEUGLU
6   ARGALAVALGLUALALEUGLNGLYGLULYS
7   ARGTYRTYRSERASPPROARGPHELEUASN
8   LEUTRPLEULYSLEUGLYARGLEUCYSASN
9   GLUPROLEUASPMETTYRSERTYRLEUHIS
10   ASNGLNGLYILEGLYVALSERLEUALAGLN
11   PHETYRILESERTRPALAGLUGLUTYRGLU
12   ALAARGGLUASNPHEARGLYSALAASPALA
13   ILEPHEGLNGLUGLYILEGLNGLNLYSALA
14   GLUPROLEUGLUARGLEUGLNSERGLNHIS
15   ARGGLNPHEGLNALAARGVALSERARGGLN
16   THRLEULEUALALEUGLULYSGLUGLUGLU
17   GLUGLUVALPHEGLUSERSER

Entity 2, Blinkin residues 212-226 15 residues - 1826.2 Da.

The peptide corresponds to Blinkin residues 212-226

1   LYSILEASPPHEASNASPPHEILELYSARG
2   LEULYSTHRGLYLYS

Samples:

sample_1: Bubr1N, [U-100% 13C; U-100% 15N], 0.25 ± 0.05 mM; Blinkin peptide 0.4 ± 0.05 mM; sodium chloride 200 ± 20 mM; sodium phosphate 50 ± 5 mM; sodium azide 1 ± 0.5 mM; TSP 0.1 ± 0.01 mM; TCEP 1 ± 0.1 mM; H2O 90%; D2O 10%

sample_d2o: Bubr1N, [U-100% 13C; U-100% 15N], 0.25 ± 0.05 mM; Blinkin peptide 0.4 ± 0.05 mM; sodium chloride 200 ± 20 mM; sodium phosphate 50 ± 5 mM; sodium azide 1 ± 0.5 mM; TSP 0.1 ± 0.01 mM; TCEP 1 ± 0.1 mM; D2O 100%

sample_conditions_1: ionic strength: 0.5 M; pH: 7; pressure: 1 atm; temperature: 310 K

sample_conditions_2: ionic strength: 0.5 M; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_2
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_d2oisotropicsample_conditions_1
3D (H)CCH-TOCSYsample_d2oisotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_d2oisotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_d2oisotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_d2oisotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection

NMRView v5.2.2_01, Johnson, One Moon Scientific - data analysis

NMRPipe v2010 release, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

UNP O60566 Q8NG31
PDB
DBJ BAD92019 BAG10575 BAG35587 BAC05691
GB AAC06260 AAC12730 AAC19118 AAC23736 AAC33435 AAF97513 AAH29373 AAI72422 AAL67803 AAM45143
REF NP_001202 XP_002753645 XP_002804771 XP_002825334 XP_003266770 NP_653091 NP_733468 XP_004056050 XP_008950270 XP_009247989
SP O60566 Q8NG31
AlphaFold Q96KM4 Q9NR92 O60566 Q8NG31

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks