BMRB Entry 17602

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments of wildtype phosphomannomutase/phosphoglucomutase
Deposition date:
2011-04-26
Original release date:
2012-08-23
Authors:
VanDoren, Steven; Akella, Sarma
Citation:

Citation: Sarma, Akella; Anbanandam, Asokan; Kelm, Allek; Mehra-Chaudhary, Ritcha; Wei, Yirui; Qin, Peiwu; Lee, Yingying; Berjanskii, Mark; Mick, Jacob; Beamer, Lesa; Van Doren, Steven. "Solution NMR of a 463-residue phosphohexomutase: domain 4 mobility, substates, and phosphoryl transfer defect."  Biochemistry 51, 807-819 (2012).
PubMed: 22242625

Assembly members:

Assembly members:
PMM-PGM, polymer, 463 residues, Formula weight is not available
MG, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: P. aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET14b_PMM/PGM

Data typeCount
13C chemical shifts1394
15N chemical shifts523
1H chemical shifts523

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Monomer1
2Mg2+2

Entities:

Entity 1, Monomer 463 residues - Formula weight is not available

1   METSERTHRVALLYSALAPROTHRLEUPRO
2   ALASERILEPHEARGALATYRASPILEARG
3   GLYVALVALGLYASPTHRLEUTHRALAGLU
4   THRALATYRTRPILEGLYARGALAILEGLY
5   SERGLUSERLEUALAARGGLYGLUPROCYS
6   VALALAVALGLYARGASPGLYARGLEUSER
7   GLYPROGLULEUVALLYSGLNLEUILEGLN
8   GLYLEUVALASPCYSGLYCYSGLNVALSER
9   ASPVALGLYMETVALPROTHRPROVALLEU
10   TYRTYRALAALAASNVALLEUGLUGLYLYS
11   SERGLYVALMETLEUTHRGLYSERHISASN
12   PROPROASPTYRASNGLYPHELYSILEVAL
13   VALALAGLYGLUTHRLEUALAASNGLUGLN
14   ILEGLNALALEUARGGLUARGILEGLULYS
15   ASNASPLEUALASERGLYVALGLYSERVAL
16   GLUGLNVALASPILELEUPROARGTYRPHE
17   LYSGLNILEARGASPASPILEALAMETALA
18   LYSPROMETLYSVALVALVALASPCYSGLY
19   ASNGLYVALALAGLYVALILEALAPROGLN
20   LEUILEGLUALALEUGLYCYSSERVALILE
21   PROLEUTYRCYSGLUVALASPGLYASNPHE
22   PROASNHISHISPROASPPROGLYLYSPRO
23   GLUASNLEULYSASPLEUILEALALYSVAL
24   LYSALAGLUASNALAASPLEUGLYLEUALA
25   PHEASPGLYASPGLYASPARGVALGLYVAL
26   VALTHRASNTHRGLYTHRILEILETYRPRO
27   ASPARGLEULEUMETLEUPHEALALYSASP
28   VALVALSERARGASNPROGLYALAASPILE
29   ILEPHEASPVALLYSCYSTHRARGARGLEU
30   ILEALALEUILESERGLYTYRGLYGLYARG
31   PROVALMETTRPLYSTHRGLYHISSERLEU
32   ILELYSLYSLYSMETLYSGLUTHRGLYALA
33   LEULEUALAGLYGLUMETSERGLYHISVAL
34   PHEPHELYSGLUARGTRPPHEGLYPHEASP
35   ASPGLYILETYRSERALAALAARGLEULEU
36   GLUILELEUSERGLNASPGLNARGASPSER
37   GLUHISVALPHESERALAPHEPROSERASP
38   ILESERTHRPROGLUILEASNILETHRVAL
39   THRGLUASPSERLYSPHEALAILEILEGLU
40   ALALEUGLNARGASPALAGLNTRPGLYGLU
41   GLYASNILETHRTHRLEUASPGLYVALARG
42   VALASPTYRPROLYSGLYTRPGLYLEUVAL
43   ARGALASERASNTHRTHRPROVALLEUVAL
44   LEUARGPHEGLUALAASPTHRGLUGLUGLU
45   LEUGLUARGILELYSTHRVALPHEARGASN
46   GLNLEULYSALAVALASPSERSERLEUPRO
47   VALPROPHE

Entity 2, Mg2+ - Mg - 24.305 Da.

1   MG

Samples:

sample_1: PMM-PGM, [U-100% 13C; U-100% 15N; U-80% 2H], 1.0 mM; D2O, [U-99.9% 2H], 5%; H2O 95%; Magnesium Chloride 1 mM; MOPS 50 mM

PMM_sample_conditions_1: ionic strength: 0.003 M; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSYsample_1isotropicPMM_sample_conditions_1
3D TROSY-HNCOsample_1isotropicPMM_sample_conditions_1
3D TROSY-HNCAsample_1isotropicPMM_sample_conditions_1
3D TROSY-HN(CO)CAsample_1isotropicPMM_sample_conditions_1
3D TROSY-HNCACBsample_1isotropicPMM_sample_conditions_1
3D TROSY-HNCACOsample_1isotropicPMM_sample_conditions_1

Software:

TOPSPIN v2.1.3, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 17652
PDB
DBJ BAK92897 BAP25224 BAP54026 BAQ43263 BAR70810
EMBL CAW30471 CCQ87804 CDH74092 CDH80443 CDI93917
GB AAA25701 AAG08707 ABJ14705 ABR82184 AEO77919
REF NP_254009 WP_003098295 WP_003114359 WP_003117161 WP_003121305
SP P26276 Q02E40
AlphaFold P26276 Q02E40

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks