BMRB Entry 17549

Title:
Backbone Resonance Assignment of the von-Willebrand-factor-A-like domain 2 of type VII collagen
Deposition date:
2011-03-24
Original release date:
2011-05-04
Authors:
Leineweber, Sarah
Citation:

Citation: Leineweber, Sarah; Schonig, Sarah; Seeger, Karsten. "Insight into interactions of the von-Willebrand-factor-A-like domain 2 with the FNIII-like domain 9 of collagen VII by NMR and SPR."  FEBS Lett. 585, 1748-1752 (2011).
PubMed: 21570975

Assembly members:

Assembly members:
mvWFA2, polymer, 196 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTWIN1

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts167
1H chemical shifts366

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mvWFA21

Entities:

Entity 1, mvWFA2 196 residues - Formula weight is not available

1   GLYARGALAMETGLYALACYSSERHISGLY
2   PROVALASPVALVALPHELEULEUHISALA
3   THRARGASPASNALAHISASNALAGLUALA
4   VALARGARGVALLEUGLUARGLEUVALSER
5   ALALEUGLYPROLEUGLYPROGLNALAALA
6   GLNVALGLYLEULEUTHRTYRSERHISARG
7   PROSERPROLEUPHEPROLEUASNSERSER
8   HISASPLEUGLYILEILELEUARGLYSILE
9   ARGASPILEPROTYRVALASPPROSERGLY
10   ASNASNLEUGLYTHRALAVALTHRTHRALA
11   HISARGTYRLEULEUALASERASNALAPRO
12   GLYARGARGGLNGLNVALPROGLYVALMET
13   VALLEULEUVALASPGLUPROLEUARGGLY
14   ASPILELEUSERPROILEARGGLUALAGLN
15   THRSERGLYLEULYSVALMETALALEUSER
16   LEUVALGLYALAASPPROGLUGLNLEUARG
17   ARGLEUALAPROGLYTHRASPPROILEGLN
18   ASNPHEPHEALAVALASPASNGLYPROGLY
19   LEUASPARGALAVALSERASPLEUALAVAL
20   ALALEUCYSGLNALAALA

Samples:

sample_1: mvWFA2, [U-100% 13C; U-100% 15N], 2 mM; potassium phosphate 10 mM; TSP 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks