BMRB Entry 17493

Title:
Solution structure of the MoCVNH-LysM module from the rice blast fungus Magnaporthe oryzae protein (MGG_03307)
Deposition date:
2011-02-26
Original release date:
2011-05-06
Authors:
Koharudin, Leonardus; Viscomi, Arturo; Montanini, Barbara; Kershaw, Michael; Talbot, Nicholas; Ottonello, Simone; Gronenborn, Angela
Citation:

Citation: Koharudin, Leonardus; Viscomi, Arturo; Montanini, Barbara; Kershaw, Michael; Talbot, Nicholas; Ottonello, Simone; Gronenborn, Angela. "Structure-Function Analysis of a CVNH-LysM Lectin Expressed during Plant Infection by the Rice Blast Fungus Magnaporthe oryzae."  Structure 19, 662-674 (2011).
PubMed: 21565701

Assembly members:

Assembly members:
entity, polymer, 167 residues, 18176.980 Da.

Natural source:

Natural source:   Common Name: rice blast fungus   Taxonomy ID: 318829   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Magnaporthe oryzae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15B

Data sets:
Data typeCount
13C chemical shifts407
15N chemical shifts168
1H chemical shifts774

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MoCVNH-LysM1

Entities:

Entity 1, MoCVNH-LysM 167 residues - 18176.980 Da.

1   GLYASNTYRALAGLYASNPHESERGLYSER
2   SERARGASPILECYSLEUASPGLYALAARG
3   LEUARGALAGLUCYSARGARGGLYASPGLY
4   GLYTYRSERTHRSERVALILEASPLEUASN
5   ARGTYRLEUSERASNASPASNGLYHISPHE
6   ARGTRPVALSERGLYGLYGLYGLYGLYGLY
7   GLYTHRALATHRVALTHRVALGLNGLNGLY
8   ASPTHRLEUARGASPILEGLYARGARGPHE
9   ASPCYSASPPHEHISGLUILEALAARGARG
10   ASNASNILEGLNASNGLUASPLEUILETYR
11   PROGLYGLNVALLEUGLNVALPROTHRLYS
12   GLYGLYSERGLYGLYGLYALAGLYASNPHE
13   TRPASPSERALAARGASPVALARGLEUVAL
14   ASPGLYGLYLYSVALLEUGLUALAGLULEU
15   ARGTYRSERGLYGLYTRPASNARGSERARG
16   ILETYRLEUASPGLUHISILEGLYASNARG
17   ASNGLYGLULEUILEHISCYS

Samples:

sample_1: MoCVNH-LysM, [U-100% 15N], 0.5 ± 0.01 mM; H2O 90%; D2O 10%; sodium acetate25 – 50 mM; NaCl 20 mM; NaN3 3 mM

sample_2: MoCVNH-LysM, [U-100% 13C; U-100% 15N], 0.5 ± 0.01 mM; H2O 90%; D2O 10%; sodium acetate25 – 50 mM; NaCl 20 mM; NaN3 3 mM

sample_conditions_1: ionic strength: 0.020 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 0.020 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_2
3D CBCA(CO)NHsample_2isotropicsample_conditions_2
3D H(CCO)NHsample_2isotropicsample_conditions_2
3D C(CO)NHsample_2isotropicsample_conditions_2
3D HCCH-TOCSYsample_2isotropicsample_conditions_2
3D 1H-15N NOESYsample_2isotropicsample_conditions_2
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_2
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView v5.2.2_01, Johnson, One Moon Scientific - data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - Automatic NOE assignment

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB EHA50335 ELQ44149 ELQ65722
REF XP_003716654

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks