BMRB Entry 17382

Title:
Solution structure of the human Raf-1 kinase inhibitor protein
Deposition date:
2011-01-27
Original release date:
2011-02-15
Authors:
Yi, C.
Citation:

Citation: Yi, Cuiying; Peng, Yu; Guo, Chenyun; Lin, Donghai. "1H, 13C, 15N backbone and side-chain resonance assignments of the human Raf-1 kinase inhibitor protein."  Biomol. NMR Assignments 5, 63-66 (2011).
PubMed: 20924725

Assembly members:

Assembly members:
Raf-1_kinase_inhibitor_protein, polymer, 187 residues, 21086.967 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pReceiver-B01a

Data sets:
Data typeCount
13C chemical shifts755
15N chemical shifts181
1H chemical shifts1241

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Raf-1 kinase inhibitor protein1

Entities:

Entity 1, Raf-1 kinase inhibitor protein 187 residues - 21086.967 Da.

1   METPROVALASPLEUSERLYSTRPSERGLY
2   PROLEUSERLEUGLNGLUVALASPGLUGLN
3   PROGLNHISPROLEUHISVALTHRTYRALA
4   GLYALAALAVALASPGLULEUGLYLYSVAL
5   LEUTHRPROTHRGLNVALLYSASNARGPRO
6   THRSERILESERTRPASPGLYLEUASPSER
7   GLYLYSLEUTYRTHRLEUVALLEUTHRASP
8   PROASPALAPROSERARGLYSASPPROLYS
9   TYRARGGLUTRPHISHISPHELEUVALVAL
10   ASNMETLYSGLYASNASPILESERSERGLY
11   THRVALLEUSERASPTYRVALGLYSERGLY
12   PROPROLYSGLYTHRGLYLEUHISARGTYR
13   VALTRPLEUVALTYRGLUGLNASPARGPRO
14   LEULYSCYSASPGLUPROILELEUSERASN
15   ARGSERGLYASPHISARGGLYLYSPHELYS
16   VALALASERPHEARGLYSLYSTYRGLULEU
17   ARGALAPROVALALAGLYTHRCYSTYRGLN
18   ALAGLUTRPASPASPTYRVALPROLYSLEU
19   TYRGLUGLNLEUSERGLYLYS

Samples:

sample_1: Raf-1 kinase inhibitor protein, [U-100% 13C; U-100% 15N], 3 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 200 mM; pH: 4.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16992 18204
PDB
DBJ BAA03684 BAE88027 BAE88359 BAG34868 BAG61396
EMBL CAA51652 CAA53031 CAA59404 CAH93256
GB AAB32876 AAD14234 AAH08714 AAH17396 AAH31102
PRF 2117380B
REF NP_001126915 NP_001233128 NP_002558 XP_002753109 XP_004054017
SP P30086 P48737 Q5R4R0
AlphaFold P30086 Q5R4R0 P48737

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks