BMRB Entry 17069

Title:
Backbone dynamics of E73 from SSV-RH
Deposition date:
2010-07-21
Original release date:
2010-08-24
Authors:
Goel, Anupam
Citation:

Citation: Schlenker, Casey; Goel, Anupam; Tripet, Brian; Menon, Smita; Lawrence, C. Martin; Valerie, Copie. "Structure and dynamics characterization of the E73 protein from Sulfolobus Spindle-shaped Virus Ragged Hills (SSV-RH), a hyperthermophilic crenarchaeal virus from Yellowstone National Park."  Biochemistry ., .-..

Assembly members:

Assembly members:
E73, polymer, 80 residues, 9578.2 Da.

Natural source:

Natural source:   Common Name: Sulfolobus spindle Virus   Taxonomy ID: 256994   Superkingdom: Virus   Kingdom: not available   Genus/species: Fusellovirus Sulfolobus spindle-shaped Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET14

Entity Sequences (FASTA):

Data sets:
Data typeCount
heteronuclear NOE values58
order parameters57
spectral density values58
T1 relaxation values58
T2 relaxation values58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1E73, chain 11
2E73, chain 21

Entities:

Entity 1, E73, chain 1 80 residues - 9578.2 Da.

1   METHISHISHISHISHISHISMETVALGLU
2   SERLYSLYSILEALALYSLYSLYSTHRTHR
3   LEUALAPHEASPGLUASPVALTYRHISTHR
4   LEULYSLEUVALSERVALTYRLEUASNARG
5   ASPMETTHRGLUILEILEGLUGLUALAVAL
6   VALMETTRPLEUILEGLNASNLYSGLULYS
7   LEUPROASNGLULEULYSPROLYSILEASP
8   GLUILESERLYSARGPHEPHEPROALALYS

Samples:

sample_relax: E73, [U-100% 13C; U-100% 15N], 5 mM; D2O 10%; H2O 90%; PMSF 0.1 mM; sodium azide 0.01%; potassium phosphate 50 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 0.005 M; pH: 5.0; pressure: 1 atm; temperature: 312 K

Experiments:

NameSampleSample stateSample conditions
2D 15N T1sample_relaxanisotropicsample_conditions_1
2D 15N T2 interleavedsample_relaxanisotropicsample_conditions_1
2D 15N {1H} nOesample_relaxanisotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

ModelFree, Palmer - data analysis, geometry optimization, refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 16177 18132
PDB
GB AAR27912
REF NP_963940