BMRB Entry 16840

Title:
1H, 15N and 13C resonance assignment of Darcin, a Major Urinary Protein of the mouse
Deposition date:
2010-04-06
Original release date:
2010-08-19
Authors:
Phelan, Marie; McLean, Lynn; Simpson, Deborah; Hurst, Jane; Beynon, Robert; Lian, Lu-Yun
Citation:

Citation: Phelan, Marie; McLean, Lynn; Simpson, Deborah; Hurst, Jane; Beynon, Robert; Lian, Lu-Yun. "1H, 15N and 13C resonance assignment of darcin, a mouse major urinary protein."  Biomol. NMR Assignments 4, 239-241 (2010).
PubMed: 20703836

Assembly members:

Assembly members:
darcin, polymer, 176 residues, 20533.9058 Da.

Natural source:

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Data sets:
Data typeCount
13C chemical shifts781
15N chemical shifts186
1H chemical shifts1202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1darcin1

Entities:

Entity 1, darcin 176 residues - 20533.9058 Da.

residues 20 to 181 correspond to the native MUP residues 6 to 19 correspond to the cloning and purification tag

1   METGLYSERSERHISHISHISHISHISHIS
2   ILEGLUGLYARGGLUGLUALASERSERMET
3   GLUARGASNPHEASNVALGLULYSILEASN
4   GLYGLUTRPTYRTHRILEMETLEUALATHR
5   ASPLYSARGGLULYSILEGLUGLUHISGLY
6   SERMETARGVALPHEVALGLUTYRILEHIS
7   VALLEUGLUASNSERLEUALALEULYSPHE
8   HISILEILEILEASNGLUGLUCYSSERGLU
9   ILEPHELEUVALALAASPLYSTHRGLULYS
10   ALAGLYGLUTYRSERVALTHRTYRASPGLY
11   SERASNTHRPHETHRILELEULYSTHRASP
12   TYRASPASNTYRILEMETILEHISLEUILE
13   ASNLYSLYSASPGLYGLUTHRPHEGLNLEU
14   METGLULEUTYRGLYARGGLUPROASPLEU
15   SERSERASPILELYSGLULYSPHEALAGLN
16   LEUSERGLUGLUHISGLYILEVALARGGLU
17   ASNILEILEASPLEUTHRASNALAASNARG
18   CYSLEUGLUALAARGGLU

Samples:

sample_1: darcin, [U-98% 13C; U-98% 15N], 1 mM; potassium phosphate 25 mM; sodium azide 0.2%; H2O 90%; D2O 10%

sample_2: darcin, [U-98% 13C; U-98% 15N], 1 mM; potassium phosphate 25 mM; sodium azide 0.2%; D2O 100%

sample_conditions_1: ionic strength: 25 mM; pH: 6.8; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D 1H-13C HSQC (aromatic)sample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

ANALYSIS v2.1.3, CCPN - chemical shift assignment, data analysis

DANGLE v1.1, CCPN - Secondary structure calculation

AZARA vany, CCPN - Spectrum processing

TOPSPIN v2.1, Bruker biospin - Data collection, Spectrum processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
EMBL CAP58483 CAQ11567
GB AAH89613 AAH92096 ACF70718
REF NP_001012323 XP_006538108
SP Q5FW60
TPG DAA06315
AlphaFold Q5FW60

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks