BMRB Entry 16522

Title:
A Doppel alpha-helix Peptide Fragment Mimics the Copper(II) Interactions with the whole Protein
Deposition date:
2009-09-28
Original release date:
2010-04-26
Authors:
Isernia, Carla; La Mendola, Diego; Magri', Antonio
Citation:

Citation: La Mendola, Diego; Magri, Antonio; Campagna, Tiziana; Campitiello, Maria Anna; Raiola, Luca; Isernia, Carla; Hansson, Orjan; Bonomo, Raffaele; Rizzarelli, Enrico. "A Doppel alpha-Helix Peptide Fragment Mimics the Copper(II) Interactions with the Whole Protein."  Chemistry 16, 6212-6223 (2010).
PubMed: 20411530

Assembly members:

Assembly members:
hDpl(122-139), polymer, 18 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hDpl(122-139): KPDNKLHQQVLWRLVQEL

Data typeCount
1H chemical shifts314
coupling constants29

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hDpl(122-139)1

Entities:

Entity 1, hDpl(122-139) 18 residues - Formula weight is not available

1   LYSPROASPASNLYSLEUHISGLNGLNVAL
2   LEUTRPARGLEUVALGLNGLULEU

Samples:

sample_1: hDpl(122-139) 1.2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 4.0; pressure: 1 atm; temperature: 300 K

sample_conditions_2: pH: 5.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_2
2D 1H-1H NOESYsample_1isotropicsample_conditions_2
2D DQF-COSYsample_1isotropicsample_conditions_2

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

VNMRJ, Varian - processing

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
DBJ BAG36200
GB AAF02424 AAG43448 AAG43449 AAH43644 AAQ89344
REF NP_036541 XP_003779349
SP Q9UKY0
AlphaFold Q9UKY0