BMRB Entry 16322

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16322

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Extracellular CD147 (EMMPRIN): Isoforms -2
Deposition date:
2009-05-28
Original release date:
2009-06-23
Authors:
Schelgel, Jennifer; Redzic, Jasmina; Porter, Christopher; Yurchenko, Vyacheslav; Bukrinsky, Michael; Armstrong, Geoffrey; Zhang, Fengli; Isern, Nancy; Degregori, James; Hodges, Robert; Eisenmesser, Elan
Citation:

Citation: Schlegel, Jennifer; Redzic, Jasmina; Porter, Christopher; Yurchenko, Vyacheslav; Bukrinsky, Michael; Labeikovsky, Wladimir; Armstrong, Geoffrey; Zhang, Fengli; Isern, Nancy; DeGregori, James; Hodges, Robert; Eisenmesser, Elan Zohar. "Solution characterization of the extracellular region of CD147 and its interaction with its enzyme ligand cyclophilin A."  J. Mol. Biol. 391, 518-535 (2009).
PubMed: 19500591

Assembly members:

Assembly members:
CD147_22-214, polymer, 198 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD147_22-214: GSHMMAAGTVFTTVEDLGSK ILLTCSLNDSATEVTGHRWL KGGVVLKEDALPGQKTEFKV DSDDQWGEYSCVFLPEPMGT ANIQLHGPPRVKAVKSSEHI NEGETAMLVCKSESVPPVTD WAWYKITDSEDKALMNGSES RFFVSSSQGRSELHIENLNM EADPGQYRCNGTSSKGSDQA IITLRVRSHLAALWPFLG

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts182
1H chemical shifts182

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Extracellular CD147 Iso-2, trans1
2Extracellular CD147 Iso-2, cis1

Entities:

Entity 1, Extracellular CD147 Iso-2, trans 198 residues - Formula weight is not available

residues 207-214 exist in both cis and trans conformations

1   GLYSERHISMETMETALAALAGLYTHRVAL
2   PHETHRTHRVALGLUASPLEUGLYSERLYS
3   ILELEULEUTHRCYSSERLEUASNASPSER
4   ALATHRGLUVALTHRGLYHISARGTRPLEU
5   LYSGLYGLYVALVALLEULYSGLUASPALA
6   LEUPROGLYGLNLYSTHRGLUPHELYSVAL
7   ASPSERASPASPGLNTRPGLYGLUTYRSER
8   CYSVALPHELEUPROGLUPROMETGLYTHR
9   ALAASNILEGLNLEUHISGLYPROPROARG
10   VALLYSALAVALLYSSERSERGLUHISILE
11   ASNGLUGLYGLUTHRALAMETLEUVALCYS
12   LYSSERGLUSERVALPROPROVALTHRASP
13   TRPALATRPTYRLYSILETHRASPSERGLU
14   ASPLYSALALEUMETASNGLYSERGLUSER
15   ARGPHEPHEVALSERSERSERGLNGLYARG
16   SERGLULEUHISILEGLUASNLEUASNMET
17   GLUALAASPPROGLYGLNTYRARGCYSASN
18   GLYTHRSERSERLYSGLYSERASPGLNALA
19   ILEILETHRLEUARGVALARGSERHISLEU
20   ALAALALEUTRPPROPHELEUGLY

Samples:

sample_1: sodium phosphate 50 mM; sodium chloride 150 mM; imidazole 50 mM; CD147 22-214, [U-99% 13C; U-99% 15N], 0.5 mM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.232 M; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian VNMRS 900 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 720 MHz

Related Database Links:

PDB
DBJ BAA08109 BAB88938 BAC67167 BAC67168 BAC76828
EMBL CAA45716 CAH92402
GB AAA68936 AAA91084 AAB41120 AAC33279 AAD10704
REF NP_001128860 NP_001719 NP_940991 NP_940992 XP_005259676
SP P35613 Q865R3
AlphaFold P35613 Q865R3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks