BMRB Entry 16318

Title:
NMR Structure of CBP TAZ2 and Adenoviral E1A Complex
Deposition date:
2009-05-27
Original release date:
2009-09-04
Authors:
Ferreon, Josephine; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter
Citation:

Citation: Ferreon, Josephine; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter. "Structural Basis for Subversion of Cellular Control Mechanisms by the Adenoviral E1A Oncoprotein"  Proc. Natl. Acad. Sci. U.S.A. 106, 13260-13265 (2009).
PubMed: 19651603

Assembly members:

Assembly members:
CBP_TAZ2, polymer, 92 residues, 10527.605 Da.
E1A, polymer, 42 residues, 4464.949 Da.
ZN, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Data sets:
Data typeCount
13C chemical shifts424
15N chemical shifts131
1H chemical shifts917

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CBP_TAZ21
2E1A2
3ZINC ION_13
4ZINC ION_23
5ZINC ION_33

Entities:

Entity 1, CBP_TAZ2 92 residues - 10527.605 Da.

1   SERPROGLNGLUSERARGARGLEUSERILE
2   GLNARGCYSILEGLNSERLEUVALHISALA
3   CYSGLNCYSARGASNALAASNCYSSERLEU
4   PROSERCYSGLNLYSMETLYSARGVALVAL
5   GLNHISTHRLYSGLYCYSLYSARGLYSTHR
6   ASNGLYGLYCYSPROVALCYSLYSGLNLEU
7   ILEALALEUCYSCYSTYRHISALALYSHIS
8   CYSGLNGLUASNLYSCYSPROVALPROPHE
9   CYSLEUASNILELYSHISLYSLEUARGGLN
10   GLNGLN

Entity 2, E1A 42 residues - 4464.949 Da.

1   SERHISMETALAPROGLUASPPROASNGLU
2   GLUALAVALSERGLNILEPHEPROASPSER
3   VALMETLEUALAVALGLNGLUGLYILEASP
4   LEULEUTHRPHEPROPROALAPROGLYSER
5   PROGLU

Entity 3, ZINC ION_1 - Formula weight is not available

1   ZN

Samples:

E1A:TAZ2: entity_1, [U-100% 13C; U-100% 15N], 1 – 1.5 mM; entity_2, [U-100% 15N], 1 – 1.5 mM; D2O 100%

E1A:TAZ2_2: entity_1, [U-100% 13C; U-100% 15N], 1 – 1.5 mM; entity_2, [U-100% 15N], 1 – 1.5 mM; Tris 20 mM; NaCl 50 mM; D2O 100%

10_mM_dTris_pH_6.8: ionic strength: 0 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

20_mM_Tris_50mM_NaCl_pH_6.8: ionic strength: 70 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCE1A:TAZ2_2isotropic20_mM_Tris_50mM_NaCl_pH_6.8
3D HNCAE1A:TAZ2isotropic10_mM_dTris_pH_6.8

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 16015
PDB
DBJ BAE06125 BAI45616
EMBL CAF96470 CAG06082 CDQ92618 CAB40663 CAB40664
GB AAB28651 AAC17736 AAC51331 AAC51340 AAC51770 AAN08102 AAQ10538 AAQ10563 AAQ19284 AAQ19285
PRF 1923401A 0512253A
REF NP_001020603 NP_001073315 NP_001088637 NP_001157494 NP_001192159 AP_000197 AP_000498
SP P45481 Q6JHU9 Q92793 P03254 P03255
TPG DAA15549
AlphaFold P45481 Q6JHU9 Q92793 P03254 P03255

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks