BMRB Entry 15690

Title:
The solution structure of the complex between MNK1 and HAH1 mediated by Cu(I)
Deposition date:
2008-03-21
Original release date:
2009-06-10
Authors:
Bertini, Ivano; Banci, Lucia; Felli, Isabella; Pavelkova, Anna; Rosato, Antonio
Citation:

Citation: Banci, Lucia; Bertini, Ivano; Calderone, Vito; Della?Malva, Nunzia; Felli, Isabella; Neri, Sara; Pavelkova, Anna; Rosato, Antonio. "Copper(I)-mediated protein-protein interactions result from suboptimal interaction surfaces."  Biochem. J. 422, 37-42 (2009).
PubMed: 19453293

Assembly members:

Assembly members:
MNK1, polymer, 73 residues, Formula weight is not available
HAH1, polymer, 68 residues, Formula weight is not available
CU1, non-polymer, 63.546 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET20

Data sets:
Data typeCount
13C chemical shifts564
15N chemical shifts137
1H chemical shifts907

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1COPPER(I) ION3
2MNK11
3HAH12

Entities:

Entity 3, COPPER(I) ION - Cu - 63.546 Da.

1   CU1

Entity 1, MNK1 73 residues - Formula weight is not available

1   METGLYVALASNSERVALTHRILESERVAL
2   GLUGLYMETTHRCYSASNSERCYSVALTRP
3   THRILEGLUGLNGLNILEGLYLYSVALASN
4   GLYVALHISHISILELYSVALSERLEUGLU
5   GLULYSASNALATHRILEILETYRASPPRO
6   LYSLEUGLNTHRPROLYSTHRLEUGLNGLU
7   ALAILEASPASPMETGLYPHEASPALAVAL
8   ILEHISASN

Entity 2, HAH1 68 residues - Formula weight is not available

1   METPROLYSHISGLUPHESERVALASPMET
2   THRCYSGLYGLYCYSALAGLUALAVALSER
3   ARGVALLEUASNLYSLEUGLYGLYVALLYS
4   TYRASPILEASPLEUPROASNLYSLYSVAL
5   CYSILEGLUSERGLUHISSERMETASPTHR
6   LEULEUALATHRLEULYSLYSTHRGLYLYS
7   THRVALSERTYRLEUGLYLEUGLU

Samples:

HAH1-Cu(I)-MNK1: MNK1 0.6 mM; HAH1, [U-100% 13C; U-100% 15N], 0.6 mM; COPPER(I) ION 0.6 mM

MNK1-Cu(I)-HAH1: MNK1, [U-100% 13C; U-100% 15N], 0.6 mM; HAH1 0.6 mM; COPPER(I) ION 0.6 mM

MNK1-Cu(I)-HAH1_excess: MNK1, [U-99% 13C; U-99% 15N], 0.6 mM; HAH1 1.0 mM; COPPER(I) ION 1.0 mM

HAH1-Cu(I)-MNK1_excess: MNK1 1.0 mM; HAH1, [U-100% 13C; U-100% 15N], 0.6 mM; COPPER(I) ION 1.0 mM

conditions_MNK1-Cu(I)-HAH1: pH: 7.0; pressure: 1 atm; temperature: 298 K

conditions_HAH1-Cu(I)-MNK1: pH: 7.0; pressure: 1 atm; temperature: 298 K

conditions_HAH1-Cu(I)-MNK1_excess: pH: 7.0; pressure: 1 atm; temperature: 298 K

conditions_MNK1-Cu(I)-HAH1_excess: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCMNK1-Cu(I)-HAH1isotropicconditions_MNK1-Cu(I)-HAH1
2D 1H-13C HSQCMNK1-Cu(I)-HAH1isotropicconditions_MNK1-Cu(I)-HAH1
3D CBCA(CO)NHMNK1-Cu(I)-HAH1isotropicconditions_MNK1-Cu(I)-HAH1
3D HNCOMNK1-Cu(I)-HAH1isotropicconditions_MNK1-Cu(I)-HAH1
3D HNCACBMNK1-Cu(I)-HAH1isotropicconditions_MNK1-Cu(I)-HAH1
3D HCCH-TOCSYMNK1-Cu(I)-HAH1isotropicconditions_MNK1-Cu(I)-HAH1
3D 1H-15N NOESYMNK1-Cu(I)-HAH1isotropicconditions_MNK1-Cu(I)-HAH1
3D 1H-13C NOESYMNK1-Cu(I)-HAH1isotropicconditions_MNK1-Cu(I)-HAH1
2D 1H-15N HSQCHAH1-Cu(I)-MNK1isotropicconditions_HAH1-Cu(I)-MNK1
2D 1H-13C HSQCHAH1-Cu(I)-MNK1isotropicconditions_HAH1-Cu(I)-MNK1
3D CBCA(CO)NHHAH1-Cu(I)-MNK1isotropicconditions_HAH1-Cu(I)-MNK1
3D HNCOHAH1-Cu(I)-MNK1isotropicconditions_HAH1-Cu(I)-MNK1
3D HNCACBHAH1-Cu(I)-MNK1isotropicconditions_HAH1-Cu(I)-MNK1
3D HCCH-TOCSYHAH1-Cu(I)-MNK1isotropicconditions_HAH1-Cu(I)-MNK1
3D 1H-15N NOESYHAH1-Cu(I)-MNK1isotropicconditions_HAH1-Cu(I)-MNK1
3D 1H-13C NOESYHAH1-Cu(I)-MNK1isotropicconditions_HAH1-Cu(I)-MNK1
13C-edited,13C-edited,13C,15N NOESYMNK1-Cu(I)-HAH1isotropicconditions_MNK1-Cu(I)-HAH1
13C-edited,13C-edited,13C,15N-filtered NOESYHAH1-Cu(I)-MNK1isotropicconditions_HAH1-Cu(I)-MNK1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 400 MHz
  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB
BMRB 6266 18299 6266
DBJ BAF85752
EMBL CAG33182
GB AAC51227 AAI12249 AAI12251 AAN84554 AAP88788
REF NP_004036 XP_001169183 XP_002816151 XP_003276598 XP_003829035
SP O00244
AlphaFold O00244

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks