BMRB Entry 15402

Title:
Calcium binding protein in the calcium-binding form
Deposition date:
2007-07-23
Original release date:
2011-05-03
Authors:
Zhang, X.; Hu, Y.; Jin, C.
Citation:

Citation: Hu, Yunfei; Zhang, Xinxin; Shi, Yunming; Zhou, Yanfeng; Zhang, Wei; Su, Xiao-Dong; Xia, Bin; Zhao, Jindong; Jin, Changwen. "Structures of Anabaena Calcium-binding Protein CcbP: INSIGHTS INTO CA2+ SIGNALING DURING HETEROCYST DIFFERENTIATION."  J. Biol. Chem. 286, 12381-12388 (2011).
PubMed: 21330362

Assembly members:

Assembly members:
Alr1010_protein, polymer, 126 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Anabaena sp.   Taxonomy ID: 1167   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Anabaena Anabaena sp.

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Data sets:
Data typeCount
13C chemical shifts538
15N chemical shifts132
1H chemical shifts819

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ccbp1
2calcium ion2

Entities:

Entity 1, ccbp 126 residues - Formula weight is not available

1   METALASERVALGLUARGASPGLUTHRARG
2   GLUHISARGILEGLUTHRGLUILEILEVAL
3   ASPALAGLUASPLYSGLUGLUARGALAMET
4   GLYTRPTYRTYRTYRLEUASPASPTHRLEU
5   GLUPHEPROPHEMETGLYLYSTRPLYSLYS
6   LYSSERARGLYSTHRSERTHRILEGLUGLU
7   LYSTHRVALGLUVALLEUGLYMETALAPRO
8   ASPASPGLUCYSLEULYSASPMETTYRVAL
9   GLUVALALAASPILEGLYGLYLYSASPASP
10   ASPVALTYRTHRALALYSLEUSERASPILE
11   GLUALAILEASPVALASPASPASPTHRGLN
12   GLUALAILEALAASPTRPLEUTYRTRPLEU
13   ALAARGGLYTYRLYSPHE

Entity 2, calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample: Alr1010 protein, [U-95% 13C; U-95% 15N], 1 mM; Tris 20 mM; NaCl 220 mM; DTT 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 260 mM; pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separated_NOESYsampleisotropicsample_conditions_1
3D_15N-separated_NOESYsampleisotropicsample_conditions_1
HNCACB,CBCA(CO)NHsampleisotropicsample_conditions_1
HNCO,HN(CA)COsampleisotropicsample_conditions_1
HC(C)H-COSYsampleisotropicsample_conditions_1
HC(C)H-TOCSYsampleisotropicsample_conditions_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v2.1, F.Delaglio, S.Grzesiek, G.W.Vuister, G.Zhu, J.Pfeifer, A.Bax - processing

NMRView v5, B.A.Johnson, R.A.Blevins - data analysis

DYANA vcyana2.0, P.Guntert, C.Mumenthaler, Wuthrich, K.W.Thrich - structure solution

AMBER v7.0, D.A.Case - refinement

NMR spectrometers:

  • Bruker AVANCE 800 MHz
  • Bruker AVANCE 500 MHz

Related Database Links:

BMRB 15401
PDB
DBJ BAB72967
GB AAX13998
REF WP_010995184

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks