BMRB Entry 15112

Title:
Assignment of the reduced Cu,Zn Superoxide Dismutase from Salmonella choleraesuis
Deposition date:
2007-01-24
Original release date:
2007-06-27
Authors:
Jimenez, Beatriz; Mori, Mirko; Piccioli, Mario; Sette, Marco; Battistoni, Andrea
Citation:

Citation: Jimenez, Beatriz; Mori, Mirko; Battistoni, Andrea; Sette, Marco; Piccioli, Mario. "NMR assignment of reduced form of copper, zinc superoxide dismutase from Salmonella enterica"  Biomol. NMR Assignments 1, 65-67 (2007).
PubMed: 19636828

Assembly members:

Assembly members:
c2sod, polymer, 154 residues, Formula weight is not available
CU1, non-polymer, Formula weight is not available
ZN, non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: Salmonella choleraesuis   Taxonomy ID: 591   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Salmonella choleraesuis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMCPromEcSOD

Data sets:
Data typeCount
13C chemical shifts679
15N chemical shifts178
1H chemical shifts1034

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1c2sod1
2Cu(I)2
3Zn(II)3

Entities:

Entity 1, c2sod 154 residues - Formula weight is not available

1   ALASERGLULYSVALGLYMETASNLEUVAL
2   THRALAGLNGLYVALGLYGLNSERILEGLY
3   THRVALVALILEASPGLUTHRGLUGLYGLY
4   LEULYSPHETHRPROHISLEULYSALALEU
5   PROPROGLYGLUHISGLYPHEHISILEHIS
6   ALAASNGLYSERCYSGLNPROALAILELYS
7   ASPGLYGLNALAVALALAALAGLUALAALA
8   GLYGLYHISLEUASPPROGLNASNTHRGLY
9   LYSHISGLUGLYPROGLUGLYGLNGLYHIS
10   LEUGLYASPLEUPROVALLEUVALVALASN
11   ASNASPGLYILEALATHRGLUPROVALTHR
12   ALAPROARGLEULYSSERLEUASPGLUVAL
13   LYSASPLYSALALEUMETILEHISVALGLY
14   GLYASPASNMETSERASPGLNPROLYSPRO
15   LEUGLYGLYGLYGLYTHRARGTYRALACYS
16   GLYVALILELYS

Entity 2, Cu(I) - Formula weight is not available

1   CU1

Entity 3, Zn(II) - Formula weight is not available

1   ZN

Samples:

sample_1: c2sod, [U-95% 13C; U-95% 15N], 1.8 mM

sample_2: c2sod, [U-98% 15N], 1.6 mM

sample_conditions_1: ionic strength: 0.02 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_2isotropicsample_conditions_1
3D HNHBsample_2isotropicsample_conditions_1
2D CACOsample_1isotropicsample_conditions_1
2D CBCACOsample_1isotropicsample_conditions_1
2D CONsample_1isotropicsample_conditions_1
2D CC-COSYsample_1isotropicsample_conditions_1

Software:

CARA vcara_1.5.3_linux, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAJ36397 BAP07292
EMBL CAD01927 CAF06532 CAR33189 CAR37537 CAR59488
GB AAC13559 AAL20362 AAO68958 AAV74622 AAV77354
PIR AF0694
REF NP_456090 NP_460403 WP_000315605 WP_000826811 WP_000826812
SP O68901
AlphaFold O68901

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks