BMRB Entry 12000

Title:
Backbone chemical shifts assignments of a two-domain family GH19 chitinase allergen from Japanese cedar (Cryptomeria japonica) pollen,CJP-4.
Deposition date:
2016-07-28
Original release date:
2017-03-07
Authors:
Takashima, Tomoya; Ohnuma, Takayuki; Fukamizo, Tamo
Citation:

Citation: Takashima, Tomoya; Ohnuma, Takayuki; Fukamizo, Tamo. "NMR assignments and ligand-binding studies on a two-domain family GH19 chitinase allergen from Japanese cedar (Cryptomeria japonica) pollen"  Biomol. NMR Assign. 11, 85-90 (2017).
PubMed: 28032262

Assembly members:

Assembly members:
CJP-4, polymer, 254 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Cryptomeria japonica   Taxonomy ID: 3369   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Cryptomeria japonica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-Blue1

Data sets:
Data typeCount
13C chemical shifts649
15N chemical shifts215
1H chemical shifts216

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CJP-41

Entities:

Entity 1, CJP-4 254 residues - Formula weight is not available

1   METHISHISHISHISHISHISGLNASNCYS
2   GLYCYSASNGLYLEUCYSCYSSERGLNTYR
3   GLYTYRCYSGLYSERGLYGLUALATYRCYS
4   GLYALAGLYCYSLYSGLUGLYPROCYSSER
5   SERSERSERPROPROSERTHRGLYTHRGLY
6   VALGLYSERILEVALSERSERASPVALPHE
7   ASNSERILEVALGLYGLYALAALASERGLY
8   CYSALAGLYASNGLYPHETYRTHRTYRASP
9   SERPHEILESERALAALAASNALAPHEASN
10   GLYPHEGLYTHRSERGLYSERSERASPVAL
11   ASNLYSARGGLUILEALAALAPHEPHEALA
12   ASNALAALAHISGLNTHRGLYGLYPHECYS
13   TYRILEGLUGLUGLNASNPROTHRSERILE
14   TYRCYSASPALASERASNTHRGLNTYRPRO
15   CYSALASERGLYLYSTHRTYRHISGLYARG
16   GLYPROLEUGLNLEUSERTRPASNTYRASN
17   TYRGLYALAALAGLYSERTYRILEGLNPHE
18   ASPGLYLEUASNASNPROGLUILEVALGLY
19   THRASPSERTHRILESERPHELYSTHRALA
20   VALTRPPHETRPMETVALASNSERASNCYS
21   HISTHRALAILETHRSERGLYGLNGLYPHE
22   GLYALATHRILEARGALAILEASNSERMET
23   GLUCYSASPGLYGLYASNALAALATHRVAL
24   ALASERARGVALASNTYRTYRGLNLYSPHE
25   CYSGLNGLNLEUASNVALASPTHRGLYSER
26   ASNLEUGLNCYS

Samples:

sample_1: CJP-4, [U-95% 13C; U-95% 15N], 0.3 mM; sodium acetate 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks